Example sentences for: pcu

How can you use “pcu” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We have carefully reexamined potential cell cycle variations of Pcu1p neddylation apparent in the IP/immunoblotting experiment in Fig.

  • The conserved "cysteine box" in Csn5p is not required for Pcu3p deneddylation

  • Appearance of multiubiquitin chains was inhibited by mutant ubiquitin lacking all lysines, indicating that they were specifically generated in the reaction rather than co-precipitated with Pcu3p-Myc (Fig.

  • For example, binding by CSN could protect Pcu3p from the Ned8p-conjugating enzyme Ubc12p, thus maintaining a fraction of Pcu3p in a deneddylated state.

  • Several lines of evidence suggest that the SCF pathway is also conserved in the fission yeast Schizosaccharomyces pombe . Pcu1p, a Cdc53p/CUL1-related protein, was shown to associate with two different F-box/WD repeat proteins, Pop1p and Pop2p, when overexpressed [ 18 ] . In addition, genetic studies demonstrated that both of these F-box proteins are simultaneously required for efficient destruction of Rum1p and the replication initiator Cdc18p [ 18 19 20 ] . Rum1p is a Sic1p-analogous CDK inhibitor, which accumulates in G1, but is degraded as cells enter S phase [ 21 22 ] . Failure to degrade Rum1p is the major phenotypic defect of pop1 and pop2 deletion strains, which leads to disturbance of normal cell cycle progression, resulting in polyploidy [ 18 19 20 23 ] . Based on the genetic data and the biochemical observation that Pop1p and Pop2p interact when overexpressed, a putative SCF Pop1p-Pop2pcomplex was proposed, which would contain the heterooligomerizing F-box proteins Pop1p and Pop2p bound to SCF core components [ 18 20 ] .


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