Words similar to pcu

Example sentences for: pcu

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Several findings indicate that the slower migrating form of Pcu3p arose from covalent modification with the ubiquitin-related protein Ned8p: First, overexpression of a fusion protein of Ned8p and glutathione transferase (GST-Ned8p) converted the faster, unmodified form of Pcu3p into a high molecular weight species that reacted with antibodies to GST (Fig.
For example, binding by CSN could protect Pcu3p from the Ned8p-conjugating enzyme Ubc12p, thus maintaining a fraction of Pcu3p in a deneddylated state.
In the next step, we confirmed, by immunoprecipitation, that both forms of Pcu3p-Myc bound endogenous Pip1p in a CSN-independent manner (Fig.
recently demonstrated that partially purified CSN promotes the cleavage of Pcu1p-Ned8p conjugates in vitro [ 10], but it remained unclear whether the enzymatic activity is contained within one of the CSN subunits or a tightly associated peptidase.
Thus, as with other S. pombe cullins [ 5], Pcu3p undergoes covalent modification by ubiquitin-like Ned8p at lysine 729.