Example sentences for: pcrs

How can you use “pcrs” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Genotyping was performed using genomic DNA, either untreated or amplified linearly using primer-extension preamplification [ 30 31 ] . Approximately 0.5 μg genomic DNA was used for preamplification with an N 15 -primer [ 30 31 ] . The resulting preamplified DNA was then used as a template in marker PCRs with specific primers.

  • As an internal control, PCR for a section of the beta chain constant region was performed in parallel with VB-specific PCRs using the following primers: UpBC (5′- CGCTGTGTTTGAGCCATC-3′) and LoBC (5′- TGCTCAGGCAGTATCTGGA-3′).

  • Except for the negative control, all VH PCRs led to a product of the expected length (350 bp).

  • PCRs were performed as described above except that 1 μl of pooled BAC library DNA was used as template and the annealing temperature for most markers was raised to 60°C to achieve greater specificity.

  • PCRs were performed in a model 9600 Thermocycler (Perkin-Elmer Cetus) using the following cycling protocol: initial denaturation at 94°C for 10 min; 94.


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