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Example sentences for: pcrs

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PCRs with a band of appropriate size were considered positive and scored as 1, negative PCRs as 0 and ambiguous PCRs with bands of unexpected size as 2. The GeneBridge 4 RH map was constructed using a publicly available server at the Whitehead Institute at the Massachusetts Institute of Technology.
All test PCRs were carried out using 30 ng of template DNA with 1.5 μl GeneAmp 10 × PCR buffer II (Roche Molecular Systems, Inc.), either 1.5 mM or 3.0 mM Mg 2+, 200 μM dNTPs, 0.2 μM each forward and reverse primer, and 0.375 units of AmpliTaq Gold Polymerase (Roche Molecular Systems, Inc.) in a 15 μl final volume.
Except for the negative control, all VH PCRs led to a product of the expected length (350 bp).
PCRs were set up using a laboratory workstation (Biomek 1000; Beckman, Palo Alto, CA) and performed in PEC 9600 thermal cyclers (Perkin Elmer Cetus, Foster City, CA).
For the additional genotyping, PCRs were carried out with one of the primers radioactively labeled.