Words similar to pcrs

Example sentences for: pcrs

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Genotyping with polymorphic di-, tri-, or tetranucleotide-repeat markers was performed by PCR according to the conditions used in the test PCRs above except that one of the primers was 32P-labeled with T4 polynucleotide kinase.
All test PCRs were carried out using 30 ng of template DNA with 1.5 μl GeneAmp 10 × PCR buffer II (Roche Molecular Systems, Inc.), either 1.5 mM or 3.0 mM Mg 2+, 200 μM dNTPs, 0.2 μM each forward and reverse primer, and 0.375 units of AmpliTaq Gold Polymerase (Roche Molecular Systems, Inc.) in a 15 μl final volume.
PCRs were performed as described above except that 1 μl of pooled BAC library DNA was used as template and the annealing temperature for most markers was raised to 60°C to achieve greater specificity.
PCRs and sequencing
For spectratyping, PCRs were performed as described above with the following VB14- and VB17-specific 5′ primers: VB14m (5′- ACCCAAGATACCTCATCACAG-3′) and VB17 (5′- GACAGGACCCAGGGCAAG-3′), followed by a run-off PCR with downstream VB-specific primers: VB14 (5′- GGGCTTAAGGCAGATCTACT-3′) and VB17m (5′- TTTCAGAAAGGAGATATAGCT-3′), and FAM6-labeled BC63 3′ primer.