Words similar to pcrs

Example sentences for: pcrs

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The ATPase 6 clones were used as templates in PCRs with primer pairs RATPase6F (5'-ACCCCCATCTCACTAATTCC-3') and RATPase6R (5'-AGTACTAGGGTAGCTCCTCC-3') to generate a 145 bp fragment to make ATPase 6 riboprobes.
For spectratyping, PCRs were performed as described above with the following VB14- and VB17-specific 5′ primers: VB14m (5′- ACCCAAGATACCTCATCACAG-3′) and VB17 (5′- GACAGGACCCAGGGCAAG-3′), followed by a run-off PCR with downstream VB-specific primers: VB14 (5′- GGGCTTAAGGCAGATCTACT-3′) and VB17m (5′- TTTCAGAAAGGAGATATAGCT-3′), and FAM6-labeled BC63 3′ primer.
The RT reaction was performed using AMV reverse transcriptase, and all PCRs were performed with a 15:1 mixture of Taq:Pfu polymerase.
PCRs and sequencing
PL and JA participated in the all aspects of the study, AL performed the real time PCRs, MH critically evaluated the results and DvB is the principal investigator.