Words similar to pcrs

Example sentences for: pcrs

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PL and JA participated in the all aspects of the study, AL performed the real time PCRs, MH critically evaluated the results and DvB is the principal investigator.
The ATPase 6 clones were used as templates in PCRs with primer pairs RATPase6F (5'-ACCCCCATCTCACTAATTCC-3') and RATPase6R (5'-AGTACTAGGGTAGCTCCTCC-3') to generate a 145 bp fragment to make ATPase 6 riboprobes.
PCRs were performed as described above except that 1 μl of pooled BAC library DNA was used as template and the annealing temperature for most markers was raised to 60°C to achieve greater specificity.
The PCRs were run in parallel (same concentrations, same volume and running on the same thermal block at the same time).
For spectratyping, PCRs were performed as described above with the following VB14- and VB17-specific 5′ primers: VB14m (5′- ACCCAAGATACCTCATCACAG-3′) and VB17 (5′- GACAGGACCCAGGGCAAG-3′), followed by a run-off PCR with downstream VB-specific primers: VB14 (5′- GGGCTTAAGGCAGATCTACT-3′) and VB17m (5′- TTTCAGAAAGGAGATATAGCT-3′), and FAM6-labeled BC63 3′ primer.