Words similar to pcrg
Example sentences for: pcrg
How can you use “pcrg” in a sentence? Here are some example sentences to help you improve your vocabulary:
Therefore, while PcrG is capable of interacting with LcrV, it differs from LcrG in its ability to block secretion of Yops in Y. pestis .
Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.
Error-prone PCR was used to mutagenize pcrG for selection of PcrG variants that could block Yops secretion in an lcrG background (KIM8-3002.
PcrG was present in the elution fraction (Fig.
To determine if PcrG and LcrV also interacted in Y. pestis , we attempted to copurify the two proteins from bacterial extracts.