Example sentences for: pcrg

How can you use “pcrg” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PcrG and His 6 -LcrV were coexpressed in a Y. pestis strain with lcrG and lcrV deleted and the cleared lysates were applied to a Talon column.

  • This result led to a genetic selection for PcrG variants that could transcomplement an lcrG defect.

  • This result suggests that a region of PcrG including amino acid 42 is capable of interaction with Y. pestis secretion components and suggests that the homologous LcrG region is likely to be involved in LcrG's secretion-blocking activity.

  • Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.

  • Because the residues of LcrG required for interaction with LcrV are strongly conserved in PcrG of P. aeruginosa (red asterisks in Fig.


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