Example sentences for: pcrg

How can you use “pcrg” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Therefore, while PcrG is capable of interacting with LcrV, it differs from LcrG in its ability to block secretion of Yops in Y. pestis .

  • Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.

  • Error-prone PCR was used to mutagenize pcrG for selection of PcrG variants that could block Yops secretion in an lcrG background (KIM8-3002.

  • PcrG was present in the elution fraction (Fig.

  • To determine if PcrG and LcrV also interacted in Y. pestis , we attempted to copurify the two proteins from bacterial extracts.

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