Words similar to pcna
Example sentences for: pcna
How can you use “pcna” in a sentence? Here are some example sentences to help you improve your vocabulary:
PCNA [ 37] and E2F-1 [ 1, 7] are two key mediators of the G1 → S transition and DNA synthesis.
For instance, they are dispensable for DNA damage-dependent activation of the Rad26/Rad3-kinase complex [ 18 ] , but are required for the Rad3-dependent phosphorylation of Chk1 [ 23 ] . Structural predictions of Hus1, Rad1 and Rad9 have led to the proposal that all three fold similarly to the subunits of Proliferating Cell Nuclear Antigen (PCNA) [ 24 25 26 ] . Consistent with these predictions, PCNA exists as a homotrimer [ 27 ] while Hus1, Rad1 and Rad9 all interact and possibly form a heterotrimer [ 25 28 ] . In addition, Rad17 has sequence similarity and physical interactions with subunits of Replication Factor C (RFC) [ 29 30 ] . Based on the RFC-PCNA paradigm, the Rad17-RFC complex may load the PCNA-like Rad9-Rad1-Hus1 (9-1-1) complex onto genomic lesions discovered by the Rad26/Rad3 complex.
For instance, we have observed that PCNA protein, which is up-regulated in Tat expressing cells, extract best with hydrophobic buffers from the nucleus, presumably due to its binding to DNA replication machinery (data not shown).
These data provide additional evidence that 4-AP exerts neither an anti-proliferative nor a pro-apoptotic influence on serum-supplemented GC cultures, because PCNA expression is a sensitive marker of GC proliferation and apoptosis [ 18 26 27 ] . The lack of 4-AP effect on GC viability is also evident from the flow cytometric analysis; the percentages of cells staining positive for propidium iodide were 8 ± 2% and 7 ± 2% in the presence and absence of drug (n = 4 samples of 10,000 cells/each from 4 GC isolations).
In the untreated cells, the cytoplasmic PCNA protein levels in TM10 and TM2H cell lines were similar, but the level of PCNA in the nuclear fraction was fourfold higher in TM2H cells compared with TM10 cells (Fig.
Loading...