Example sentences for: pce

How can you use “pce” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • We suspect that expression levels of the Pct1 or Pce1 enzymes in these strains exceeded a threshold required for cell viability.

  • The pce1::kanMX cassette was excised from PCE1Δ by digestion with Aat II and Nde I. The cassette fragments were gel-purified and then used to transform diploid S. pombe.

  • These results show that the pct1Δ and pce1Δ strains are viable if the chromosomal deletions are complemented by an extrachromosomal triphosphatase or guanylyltransferase gene.

  • Genomic DNA was prepared from individual isolates and the integration of the pct1::kanMX or pce1::kanMX cassettes into the correct locus was tested by PCR using diagnostic primers.

  • Expression of the capping enzymes from these plasmids is driven by the nmt1* promoter [ 19 ] . The plasmids were transformed into heterozygous pct1 +/ pct1::kanMX or pce1 +/ pct1::kanMX diploids using the lithium acetate method [ 18 ] . The Leu +diploid transformants were then sporulated on ME plates at room temperature.


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