Words similar to pce

Example sentences for: pce

How can you use “pce” in a sentence? Here are some example sentences to help you improve your vocabulary:

Genomic DNA was prepared from individual isolates and the integration of the pct1::kanMX or pce1::kanMX cassettes into the correct locus was tested by PCR using diagnostic primers.
pct1 +or pce1 +; L4, a 20-mer corresponding to the antisense-strand sequence of the 3'-flanking region ~1 kb downstream of stop codon of pct1 +or pce1 +. In the first-stage PCR, a 5'-flanking fragment was synthesized using S. pombe genomic DNA as the template and LI plus L2 as primers.
The pce1::kanMX cassette was excised from PCE1Δ by digestion with Aat II and Nde I. The cassette fragments were gel-purified and then used to transform diploid S. pombe.
The intron-less pce1 +gene was amplified from plasmid pl32-PCE1 [ 12 ] . The PCR products were digested with Xho I and Bam HI and then inserted into the S. pombe expression vector pREP41X ( LEU2 ars1 +) [ 19 ] . The inserts were sequenced to exclude the acquisition of unwanted mutations during the amplification and cloning steps.
The pct1 +and pce1 +cDNAs were cloned separately into the S. pombe expression vector pREP41X ( LEU2 ars1 +) so as to place them under the control of the nmt1* promoter.