Example sentences for: pce

How can you use “pce” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The plasmids were introduced into heterozygous pct1 +/ pct1::kanMX or pce1 +/ pce1::kanMX diploids.

  • pct1 +or pce1 +; L4, a 20-mer corresponding to the antisense-strand sequence of the 3'-flanking region ~1 kb downstream of stop codon of pct1 +or pce1 +. In the first-stage PCR, a 5'-flanking fragment was synthesized using S. pombe genomic DNA as the template and LI plus L2 as primers.

  • We suspect that expression levels of the Pct1 or Pce1 enzymes in these strains exceeded a threshold required for cell viability.

  • These results show that the pct1Δ and pce1Δ strains are viable if the chromosomal deletions are complemented by an extrachromosomal triphosphatase or guanylyltransferase gene.

  • Here we constructed pct1Δ and pce1Δ plasmids containing 5' and 3' flanking genomic sequences in which the entire triphosphatase or guanylyltransferase coding sequence was deleted and replaced by the kanamycin resistance gene [ 17 ] . The pct1::kanMX and pce1::kanMX constructs were transformed separately into a diploid strain of S. pombe and chromosomal integrants containing one copy of the wild-type gene and one of pct1::kanMX or pce1::kanMX were selected on medium containing G418.


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