Words similar to pcdna
Example sentences for: pcdna
How can you use “pcdna” in a sentence? Here are some example sentences to help you improve your vocabulary:
The genes were amplified by PCR and cloned into pcDNA3 enabling in vitro transcription.
YZ4 was then subcloned into the eukaryotic expression vector pcDNA3 (Invitrogen) and the resultant plasmid, pcDNA3/REP 2 , was transfected into COS-1 using lipofectamine according to the manufacturer's directions (Life Science Technologies) with 12 μg plasmid DNA and 45 μL lipofectamine solution.
This fragment was excised with Not I and subcloned into the Not I site of the mammalian expression vector pcDNA3 (Invitrogen), in either the sense or antisense orientation.
The rabbit PP1α cDNA in pDR540 [ 34 ] was ligated into pcDNA3 (Invitrogen) to create a fusion encoding 6 histidines (6-His) followed by an epitope (YPYDVPDYA) from the Hemophilus influenza hemagglutinin protein (HA).
1/His-p35-C/EBPβ was constructed by inserting an 1,739 bp EcoRI fragment from pRSETB-EFII into an EcoRI cut pcDNA3.
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