Example sentences for: pcdna

How can you use “pcdna” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cDNA encoding the rat α s [ 33 ] , modified to carry the EE epitope [ 34 ] , was in pcDNA1/Amp (Invitrogen).

  • Briefly, approximately 10 8cells were transfected with pcDNA 3.1 constructs (Invitrogen) bearing either murine Smo (wild-type or W539L mutant), GFP, rat β2AR (Invitrogen), or murine Ptc cDNAs using Fugene 6 (Roche).

  • Human MYOC cDNA was cloned into the pcDNA3 (Invitrogen) mammalian expression vector as described [ 12 ] . Human myocilin signal sequence deletions were generated by PCR.

  • YZ4 was then subcloned into the eukaryotic expression vector pcDNA3 (Invitrogen) and the resultant plasmid, pcDNA3/REP 2 , was transfected into COS-1 using lipofectamine according to the manufacturer's directions (Life Science Technologies) with 12 μg plasmid DNA and 45 μL lipofectamine solution.

  • The genes were amplified by PCR and cloned into pcDNA3 enabling in vitro transcription.


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