Example sentences for: pcdna

How can you use “pcdna” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To subclone 4EBP-1-5A into pEGSH the plasmid pcDNA3.

  • The PCR amplified product was digested with HindIII and XhoI and subcloned into the correspondingly digested EE-α i2 -pcDNA3.

  • Briefly, approximately 10 8cells were transfected with pcDNA 3.1 constructs (Invitrogen) bearing either murine Smo (wild-type or W539L mutant), GFP, rat β2AR (Invitrogen), or murine Ptc cDNAs using Fugene 6 (Roche).

  • The complete SKP2 cDNA or a mutant lacking the F-box (SKP2-ΔF) were cloned into a modified pcDNA3 plasmid providing an N-terminal Myc epitope tag and the human lamin B leader sequence.

  • Human MYOC cDNA was cloned into the pcDNA3 (Invitrogen) mammalian expression vector as described [ 12 ] . Human myocilin signal sequence deletions were generated by PCR.


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