Words similar to pcdna

Example sentences for: pcdna

How can you use “pcdna” in a sentence? Here are some example sentences to help you improve your vocabulary:

The complete SKP2 cDNA or a mutant lacking the F-box (SKP2-ΔF) were cloned into a modified pcDNA3 plasmid providing an N-terminal Myc epitope tag and the human lamin B leader sequence.
PCR and processing were as described above with the exception that the 5' human MYOC deletion products were cloned into a HindIII/Bsu36I digested pcDNA3.hMYOC wild-type vector.
The rabbit PP1α cDNA in pDR540 [ 34 ] was ligated into pcDNA3 (Invitrogen) to create a fusion encoding 6 histidines (6-His) followed by an epitope (YPYDVPDYA) from the Hemophilus influenza hemagglutinin protein (HA).
This fragment was excised with Not I and subcloned into the Not I site of the mammalian expression vector pcDNA3 (Invitrogen), in either the sense or antisense orientation.
A signal peptide cleavage site was predicted to exist between rabbit aa 18 and 19 (human aa 32 and 33) [ 33 ] . We directly tested the importance of the signal peptide in myocilin secretion by replacing the first 32 aa of human myocilin with a single methionine (pcDNA3.hMYOC.ΔN32).