Example sentences for: pcdna

How can you use “pcdna” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • YZ4 was then subcloned into the eukaryotic expression vector pcDNA3 (Invitrogen) and the resultant plasmid, pcDNA3/REP 2 , was transfected into COS-1 using lipofectamine according to the manufacturer's directions (Life Science Technologies) with 12 μg plasmid DNA and 45 μL lipofectamine solution.

  • Transfected plasmid DNA included wild-type human MYOC (pcDNA3.hMYOC; [ 12 ] ), wild-type rabbit MYOC (pcDNA3.RbMYOC.ex1-3), N-terminal 14 amino acid deleted human MYOC (pcDNA3.hMYOC.ΔN14), N-terminal 32 amino acid deleted human MYOC (pcDNA3.hMYOC.ΔN32), or N57S human MYOC (pcDNA3.hMYOC.N57S).

  • The cDNA encoding the rat α s [ 33 ] , modified to carry the EE epitope [ 34 ] , was in pcDNA1/Amp (Invitrogen).

  • Full-length Rlk cDNA expression constructs pCIR, pcDNA3Rlk and Rlk-GFP have been described previously [ 19].

  • To examine the in vivo function of these in vitro phosphorylations, wtp53 and p53 with the S33A and S37A mutations were sub-cloned into the expression vector pcDNA3.


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