Words similar to pcdna
Example sentences for: pcdna
How can you use “pcdna” in a sentence? Here are some example sentences to help you improve your vocabulary:
A signal peptide cleavage site was predicted to exist between rabbit aa 18 and 19 (human aa 32 and 33) [ 33 ] . We directly tested the importance of the signal peptide in myocilin secretion by replacing the first 32 aa of human myocilin with a single methionine (pcDNA3.hMYOC.ΔN32).
PCR was performed as above and PCR products digested with BamH1 (exons 1-2) or Not1 (exon 3) and processed/cloned as above to create a directional clone of rabbit MYOC exons 1-2 and 3 spliced together (pcDNA3.RbMYOCex1-3).
YZ4 was then subcloned into the eukaryotic expression vector pcDNA3 (Invitrogen) and the resultant plasmid, pcDNA3/REP 2 , was transfected into COS-1 using lipofectamine according to the manufacturer's directions (Life Science Technologies) with 12 μg plasmid DNA and 45 μL lipofectamine solution.
The cDNA encoding the rat α s [ 33 ] , modified to carry the EE epitope [ 34 ] , was in pcDNA1/Amp (Invitrogen).
pcDNA3.