Example sentences for: pcdna

How can you use “pcdna” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 1/His-p35-C/EBPβ was constructed by inserting an 1,739 bp EcoRI fragment from pRSETB-EFII into an EcoRI cut pcDNA3.

  • The cDNA encoding the rat α s [ 33 ] , modified to carry the EE epitope [ 34 ] , was in pcDNA1/Amp (Invitrogen).

  • Human TTP cDNA was generated by RT-PCR from THP-1 RNA and cloned into the pcDNA 3.1His-C vector (Invitrogen, Carlsbad, CA, USA), which encodes a histidine and epitope (EXPRESS-TAG) at the N-terminus of the protein.

  • A signal peptide cleavage site was predicted to exist between rabbit aa 18 and 19 (human aa 32 and 33) [ 33 ] . We directly tested the importance of the signal peptide in myocilin secretion by replacing the first 32 aa of human myocilin with a single methionine (pcDNA3.hMYOC.ΔN32).

  • pcDNA3/Elk-1(1-209) and pcDNA3/Elk-1(1-140) were constructed by using the Erase-A-Base system (Promega) with pcDNA3/Elk-1 (gift of J. Schwartz, Univ.


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