Words similar to pbs
Example sentences for: pbs
How can you use “pbs” in a sentence? Here are some example sentences to help you improve your vocabulary:
BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
Tissue sections were processed for ERα and ERβ immunoexpression using indirect single color immunoperoxidase immunohistochemistry [ 15 ] . For ERβ, the slides were incubated at 4°C overnight with affinity purified goat-anti human ERβ polyclonal antibody - ERβ (N-19): sc6820 raised against a peptide mapping at the amino terminus of ERβ of human origin (Santa Cruz Biotechnology, Santa Cruz, CA) diluted 1:20 in PBS.
Cell lysates were obtained by rinsing 12-day old neuronal cell cultures with ice-cold phosphate-buffered saline prior to trituration in ice-cold lysis buffer (PBS supplemented with 1% Igepal, 0.5% sodium deoxycholate, 0.1% SDS, 100 μg/ml PMSF and 300 μg/ml aprotinin).
After 20 min at 20°C, cells were washed and fixed in 1% formaldehyde in PBS.
The samples were rinsed successively in PBS and deionized water and then were mounted on glass slides in Aqua Polymount from Polysciences, Inc. (Warrington, PA).