Example sentences for: pbs

How can you use “pbs” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After washing twice with 1.0 ml PBS, the cells were resuspended in 50 μl of TdT buffer containing 0.2 M potassium cacodylate, 2.5 mM Tris-HCL (pH 6.6), 2.5 mM CoCl 2 , 0.25 mg/ml bovine serum albumin, 5.0 units of terminal deoxynucleotidyl transferase and 0.5 nM biotinylated deoxyuridine triphosphate (b-UTP) and incubated at 37°C for 30 minutes.

  • Slides of metaphase spreads were treated with 2% paraformaldehyde/PBS then rinsed with 0.2% glycine / PBS, PBS and water then air-dried.

  • NIH3T3 cells suspended in PBS were treated with 1 μM staurosporine, 1 μM calyculin A or 100 μM pervanadate and fractionated with 0.5% Triton X-100 or DOTMAC.

  • Sections of fetal and residual maternal ovarian tissues were incubated for 30 min with 10% normal goat serum and for 1 h with primary antibodies (1 μg/ml), washed in two changes of PBS, incubated for 30 min with secondary goat antirabbit (F0382) or antimouse (F0257) immunoglobulin G-fluorescein isothiocyanate (FITC) (1:40), and washed in two changes of PBS.

  • The labelled probe molecules were detected with rhodamine anti-digoxigenin (Oncor, Inc., Gaithersburg, MD) and the slides were washed 3 times in PBS and 0.1 % Nonidet NP-40.


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