Words similar to pbmcs
Example sentences for: pbmcs
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IL-2 target genes in human PBMCs and T cells This file contains expression data for IL-2-regulated probe sets detected on the Affymetrix U95Av2 array, using samples prepared from human PBMCs and purified T cells.
The PBMCs were pre-activated with anti-CD3 for 72 hr, the T cells purified, and rested for 36-40 hr prior to restimulation for 4 hr with 1 nM IL-2.
To identify the maximal number of IL-2-regulated genes, PBMCs comprised of ~80% CD3+ lymphocytes, with smaller fractions of monocytes, B cells and NK cells (Table 1) were examined.
Cells were isolated directly from PBMCs from patient 422 by FACS as described above.
Using oligonucleotide arrays of known genes to identify IL-2-regulated genes in human lymphocytes, a total of 72 unique genes were found to change expression within 4 hr of IL-2 stimulation of PBMCs.