Words similar to pbmcs
Example sentences for: pbmcs
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Clones confirmed to be tetramer-positive were expanded in T-25 flasks containing irradiated JY cells and fresh PBMCs in 25 ml of CTL medium containing PHA.
For PBMC preparations, blood samples were shipped overnight at ambient temperature to a central processing facility (Quest Diagnostics, Inc., Collegeville, PA, USA) for PBMC isolation via Ficoll gradient method [ 15 ] . Purified PBMCs were shipped in RNA lysis buffer (Clontech, Palo Alto, CA, USA) to SUGEN where isolation of total RNA was performed.
While these results supported looking at the easily accessible peripheral blood mononuclear cells (PBMCs) for potential markers that reflect the disease, some doubts persisted.
Notably, all of the other genes (22) were suppressed by IL-2 in the PBMCs, as they were in the T cells, although they did not meet the significance value of p < 0.05 in the PBMCs.
Consistent with our microarray results on CCR5 up-regulation, experiments performed in peripheral blood mononuclear cells (PBMCs) with soluble Tat has shown selective entry and replication of CCR5 virus into cells [ 27 28 ] . Up-regulation of HIV-1 coreceptor by Tat has also been reported, where a synthetic Tat protein that was immobilized on a solid substrate, up-regulated the surface expression of the chemokine receptors in purified populations of primary resting CD4+ T cells.