Words similar to pbluescript

Example sentences for: pbluescript

How can you use “pbluescript” in a sentence? Here are some example sentences to help you improve your vocabulary:

The CaCET1 gene was disrupted by insertion of a UAU1 cassette [ 16 ] . We first constructed plasmid pKS-5'3'CaCET1, in which a 665-bp PCR fragment derived from the 5' end of the CaCET1 gene (from nucleotides -50 to +615 of the open reading frame, with the A residue of the ATG translation start codon defined as position +1) was cloned between the Kpn I and Xba I sites of pBluescript KS +and a 720-bp fragment extending from position +1518 of the 1560-nt CaCET1 coding sequence into the 3' flanking genomic region was inserted between the Sac I and Sac II sites of pBluescript KS +The 3.8-kbp UAU1 gene was excised from pBME101 with Xba I and Sac II and inserted between the Xba I and Sac II sites of pKS-5'3'CaCET1 to yield pCaCET1::UAU1.
To inactivate the γ 1 gene, a 4.4 kb Bam HI/ Xho I fragment upstream of exon 1 was subcloned into a multiple cloning site 5' of the neo and Herpes virus TK gene that was in pBluescript (Stratagene).
To make double stranded RNA for RNAi, we placed the Pst I- Rsa I fragment from pJC3 into pBluescript II KS+.
Each of these PCR products was cloned individually into the Eco RV site of pBluescript KS+, and then restriction digested with Eco RI and Not I (first-set fragment) or Not I and Pst I (second-set fragment).
The specific probes were prepared by digesting the appropriate pBluescript plasmid with XbaI and EcoRI , separating the fragment on a 1.5% agarose gel followed by extraction using the Qiaex II extraction kit (Qiagen).