Words similar to pbluescript

Example sentences for: pbluescript

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To make double stranded RNA for RNAi, we placed the Pst I- Rsa I fragment from pJC3 into pBluescript II KS+.
We digested previously isolated lambda genomic clones of the human FGF8 region [ 22 ] with restriction endonucleases (Promega, Madison, WI) and analyzed them by Southern blotting using a mouse Npm3 cDNA [ 21 ] probe, as previously described [ 20 ] . An 8.4-kb Xho I fragment from these clones that hybridized to the probe was subcloned into pBluescript (Stratagene) for analysis.
The CaCET1 gene was disrupted by insertion of a UAU1 cassette [ 16 ] . We first constructed plasmid pKS-5'3'CaCET1, in which a 665-bp PCR fragment derived from the 5' end of the CaCET1 gene (from nucleotides -50 to +615 of the open reading frame, with the A residue of the ATG translation start codon defined as position +1) was cloned between the Kpn I and Xba I sites of pBluescript KS +and a 720-bp fragment extending from position +1518 of the 1560-nt CaCET1 coding sequence into the 3' flanking genomic region was inserted between the Sac I and Sac II sites of pBluescript KS +The 3.8-kbp UAU1 gene was excised from pBME101 with Xba I and Sac II and inserted between the Xba I and Sac II sites of pKS-5'3'CaCET1 to yield pCaCET1::UAU1.
After first- and second-strand synthesis, the full-length cDNAs were selected, size fractionated and cloned into pBluescript.
Each PCR product was gel-purified and cloned into the Eco RV site of pBluescript KS+ (Stratagene).