Example sentences for: pbluescript

How can you use “pbluescript” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The vector for all constructs was pBluescript(-) (Stratagene).

  • After first- and second-strand synthesis, the full-length cDNAs were selected, size fractionated and cloned into pBluescript.

  • RNase protection assays were performed as described previously [ 22 ] . A Hind III fragment of the rabbit EP 2 receptor located in the the 3' untranslated region (3'UTR) was subcloned into pBluescript SK(-) plasmid (Stratagene).

  • A 240 bp DNA fragment from S. cerevisiae ARS1 chromosomal DNA replication origin containing all the key elements including boxes A, B1, B2 and B3 was subcloned in pBlueScript KS(+) between Eco RI and Hin dIII.

  • Each of these PCR products was cloned individually into the Eco RV site of pBluescript KS+, and then restriction digested with Eco RI and Not I (first-set fragment) or Not I and Pst I (second-set fragment).


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