Words similar to pbluescript

Example sentences for: pbluescript

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The rat VEGF probe is a 0.9 kb cDNA Pst I/Sma I insert cloned into a pBluescript II KS+ vector [ 33 ] . Prehybridization and hybridization were performed in 50% formamide, 5×SSC (20×SSC is 0.3 M sodium chloride, 0.3 < sodium citrate), 10× Denhardt's solution (50× Denhardt's solution is 2% Ficoll, 2% polyvinyl pyrrolidone, 2% Bovine Serum Albumin Factor V), 50 mM sodium phophate (pH 7.0), 1% sodium dodecyl sulfate (SDS), and 250 μg/ml salmon sperm DNA at 42°C.
To inactivate the γ 1 gene, a 4.4 kb Bam HI/ Xho I fragment upstream of exon 1 was subcloned into a multiple cloning site 5' of the neo and Herpes virus TK gene that was in pBluescript (Stratagene).
The resulting DNA fragment was purified by agarose gel electrophoresis, cleaved with Eco R I, and subcloned into pBluescript KS-.
After first- and second-strand synthesis, the full-length cDNAs were selected, size fractionated and cloned into pBluescript.
The specific probes were prepared by digesting the appropriate pBluescript plasmid with XbaI and EcoRI , separating the fragment on a 1.5% agarose gel followed by extraction using the Qiaex II extraction kit (Qiagen).