Words similar to pbluescript

Example sentences for: pbluescript

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Hybridization probes were derived from a ~600-bp cDNA that contains the entire coding region of the human VEGF 165 [ 8 ] subcloned into the pBluescript II KS+ vector (Stratagene, La Jolla, CA).
The plasmid containing FS was constructed by sub-cloning of the XbaI-PstI fragment derived for rat FS cDNA clone [ 9 ] , pROF304 into pBluescript SK+.
RNase protection assays were performed as described previously [ 22 ] . A Hind III fragment of the rabbit EP 2 receptor located in the the 3' untranslated region (3'UTR) was subcloned into pBluescript SK(-) plasmid (Stratagene).
The resulting PCR product was cloned into the Eco RV site of pBluescript KS+ and clones were screened for the correct orientation.
Purified TOP1 was pre-incubated with GST or GST-BTBD2 for 20 min before addition of the DNA substrate, which was either an end-labeled 36 bp fragment containing a single strong TOP1 cleavage site [ 24 ] , or a 161 bp PvuII-HindIII fragment of pBluescript (Stratagene) [ 25 ] ). End labeling of the DNA substrates was as previously described [ 24 25 ] . Cleavage reactions were continued for 20 min before stopping with 0.5% SDS.