Example sentences for: pbluescript

How can you use “pbluescript” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All PCR products were cloned into pBluescript SK+ and their DNA sequences confirmed.

  • Purified TOP1 was pre-incubated with GST or GST-BTBD2 for 20 min before addition of the DNA substrate, which was either an end-labeled 36 bp fragment containing a single strong TOP1 cleavage site [ 24 ] , or a 161 bp PvuII-HindIII fragment of pBluescript (Stratagene) [ 25 ] ). End labeling of the DNA substrates was as previously described [ 24 25 ] . Cleavage reactions were continued for 20 min before stopping with 0.5% SDS.

  • The vector for all constructs was pBluescript(-) (Stratagene).

  • cDNA templates were subcloned into the pBluescript KS-plasmid and completely sequenced from both strands.

  • A mouse nocturnin cDNA fragment (450 bp) cloned into the pBluescript KS (+) vector (Stratagene) was linearized with XhoI or XbaI for antisense or sense probes, and radiolabeled using T7 or T3 RNA polymerase respectively.


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