Example sentences for: pbluescript

How can you use “pbluescript” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A mouse nocturnin cDNA fragment (450 bp) cloned into the pBluescript KS (+) vector (Stratagene) was linearized with XhoI or XbaI for antisense or sense probes, and radiolabeled using T7 or T3 RNA polymerase respectively.

  • Each population of cDNA was ligated into a pBluescript vector that had been digested with BamHI and alkaline phosphatased to decrease the rate of self-ligation.

  • Each PCR product was gel-purified and cloned into the Eco RV site of pBluescript KS+ (Stratagene).

  • The resulting DNA fragment was purified by agarose gel electrophoresis, cleaved with Eco R I, and subcloned into pBluescript KS-.

  • The resulting fragment was subcloned into the same sites of pBlueScript II KS (+).

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