Words similar to pbluescript

Example sentences for: pbluescript

How can you use “pbluescript” in a sentence? Here are some example sentences to help you improve your vocabulary:

The KIAA1194 cDNA was first 3'-truncated upon restriction with Bsm I (nt 3642) and Sac II (in pBluescript II SK+ vector), Klenow treatment and self-ligation, to remove undesirable Eco NI restriction sites.
All PCR products were cloned into pBluescript SK+ and their DNA sequences confirmed.
The plasmid containing FS was constructed by sub-cloning of the XbaI-PstI fragment derived for rat FS cDNA clone [ 9 ] , pROF304 into pBluescript SK+.
The resulting fragment was subcloned into the same sites of pBlueScript II KS (+).
Purified TOP1 was pre-incubated with GST or GST-BTBD2 for 20 min before addition of the DNA substrate, which was either an end-labeled 36 bp fragment containing a single strong TOP1 cleavage site [ 24 ] , or a 161 bp PvuII-HindIII fragment of pBluescript (Stratagene) [ 25 ] ). End labeling of the DNA substrates was as previously described [ 24 25 ] . Cleavage reactions were continued for 20 min before stopping with 0.5% SDS.