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Example sentences for: pbigt

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ECFP or EYFP was inserted into pBigT, and the resulting sequence was inserted into a vector containing ROSA26 genomic sequences to permit homologous recombination (Fig 1A).
To make pBigT, plasmid pSAβgeo was digested with ClaI and XbaI to remove the βgeo and bpA sequence but leave behind the SA, the ends were Klenow filled and the plasmid self-ligated.
The plasmid pBigT consists of the adenovirus splice acceptor sequence followed by a loxP site, neo expression cassette, strong transcriptional stop sequence (triple SV40 polyadenylation sequence), another loxP site in the same orientation as the first, a multiple cloning site (MCS), and the bovine growth hormone polyadenylation sequence.
The resulting plasmid was called pBigT, and its MCS contains sites for the restriction enzymes NheI, SalI, AccI, XhoI, ApaI, SacII, NotI, SacI and BclI.
To make the ROSA26 genomic sequence compatible with the pBigT plasmid, pROSA26-1 was digested with XbaI, Klenow filled and a linker (PacI, SwaI, AscI) inserted.