Words similar to pbs-tween

Example sentences for: pbs-tween

How can you use “pbs-tween” in a sentence? Here are some example sentences to help you improve your vocabulary:

The blots were blocked in 5% fat-free milk in PBS-Tween at room temperature for 1 hour, then exposed to primary antibodies (mouse anti-human MMP-1, MMP-2, MMP-3 or MMP-9 antibodies; Calbiochem, Cambridge, MA, USA), and subsequently detected using HRP conjugated rabbit anti-mouse IgG (ICN Biomedical, Costa Mesa, CA, USA) in conjunction with an enhanced chemiluminescence detection system (ECL, Amersham Pharmacia Biotech, Little Chalfont, Buckinghamshire, England).
After washing 3x with PBS-Tween (DPTC-treated PBS for the mRNA), plates are probed with an anti-M13 antibody conjugated to horseradish peroxidase (1:3000 in PBS-NFM) for 1 hour at room temperature followed by addition of 100 μl of ABTS for 15-30 minutes at room temperature.
The pegs were then immersed for 2 h in a trough containing 14 ml of anti-human IgG F(ab'2) fragment conjugated to alkaline phosphatase in PBS-Tween and developed with nitrophenyl phosphate (Sigma Chemical Co.
After overnight incubation, excess IgG antibody from the serum was washed away by thrice transferring the pegs to troughs containing 14 ml of PBS containing 0.05 % Tween 20 (PBS-Tween) for 5 min each time.