Example sentences for: pab

How can you use “pab” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The plasmid encoding the ATF-BPTI (pAB) was provided by P. Quax (TNO-PG, Leiden).

  • Western analysis with anti-GFAP pAB, SMI-31 mAB, RT-97 mAB, and anti-lamins mAB was performed on F98 cell extracts subjected to SDS-PAGE.

  • Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).

  • For instance, viruses including Herpes simplex virus type 1 (HSV-1) have been known to induce severe alterations of the host translational apparatus, including the up-regulation of ribosomal proteins and the progressive association of several nonribosomal proteins, such as VP19C, VP26, and the poly(A)-binding protein 1 (PAB1P) to ribosomes [ 39 ] . In the case of HIV-1, approximately one infectious HIV-1 genome in an infected cell could be transcribed and translated into 50,000 to 100,000 physical particles [ 40 ] . This poses an immense challenge for the virus to be able to transcribe, splice, transport, and translate its RNA into fully packaged virions in a timely fashion.

  • Antibodies p53 (PAb1801) and pan- ras (F132-62) were purchased from Oncogene Science, Cambridge, MA.


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