Example sentences for: pab

How can you use “pab” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples of known numbers of cells from the cell lines and recombinant, purified Tag prepared from a baculovirus lysate [ 40 ] were loaded on 10% polyacrylamide discontinuous mini-gels (Bio-Rad, Hercules, CA) and electrophoresed conventionally [ 41 ] . Gels were then electrophoretically blotted [ 42 ] onto PVDF membrane (Bio-Rad) without methanol for 15 min at 100 V. Tag was visualized by immunostaining with PAb416 and alkaline phosphatase conjugated secondary antibody (Sigma) using chemiluminescent detection with CDP-Star substrate according to the manufacturers directions (Tropix, Bedford, MA).

  • Fixed samples were immunostained with anti-Tag, PAb416 (Ab2, Oncogene Sciences, Mineola, NY), and fluorescein isothiocyanate (FITC) conjugated secondary antibody followed by RNase treatment and propidium staining at 50 μg/ml, as previously described [ 13 ] . Cells were excited with the 488 nm line of the argon laser of a Coulter ESP Elite Cytometer (Coulter Electronics, Miami, FL).

  • For comparison, lane 9 shows a distinct single band revealed by ERβ N-19 polyclonal antibody at ~52 kDa (52β) in time 0 trophoblast culture (T:0, ERβ pAb).

  • Bacteria: Aae, Aquifex aeolicus; Bap, Buchnera aphidicola; Bbu, Borrelia burgdorferi; Bsu, Bacillus subtilis; Bhal, Bacillus halodurans; Cje, Campylobacter jejuni; Cpn, Chlamydophila pneumoniae; Ctr, Chlamydia trachomatis; Dra, Deinococcus radiodurans; Eco, Escherichia coli; Hin, Haemophilus influenzae; Hpy, Helicobacter pylori; Mge, Mycoplasma genitalium; Mpn, Mycoplasma pneumoniae; Mtu, Mycobacterium tuberculosis; Nme, Neisseria meningitidis; Pae, Pseudomonas aeruginosa; Rpr, Rickettsia prowazekii; Syn, Synechocystis sp.; Tma, Thermotoga maritima; Tpa, Treponema pallidum; Vch, Vibrio cholerae; Xfa, Xylella fastidiosa . Eukaryote: Sce, Saccharomyces cerevisiae . Archaea: Ape, Aeropyrum pernix; Afu, Archaeoglobus fulgidus; Hbs, Halobacterium sp.; Mja, Methanococcus jannaschii; Mth, Methanobacterium thermoautotrophicum; Pho, Pyrococcus horikoshii; Pab, Pyrococcus abyssi; Tac, Thermoplasma acidophilum .

  • Western analysis with anti-GFAP pAB, SMI-31 mAB, RT-97 mAB, and anti-lamins mAB was performed on F98 cell extracts subjected to SDS-PAGE.

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