Example sentences for: paci

How can you use “paci” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A PacI site had been introduced into an EcoRI site in the exon encoding the second LIM domain of Isl1.

  • The XbaI site used for insertion into the ROSA26 genomic locus [ 4] was replaced by a linker (PacI, SwaI, AscI), so that it could be digested with PacI and AscI, and receive the bigT sequence.

  • A cassette coating IRES Cre SV40 pA and pgk-neomycin was cloned into this PacI site to create a targeting construct with flanking 5' and 3' genomic DNA arms of 5 kb and 2 kb, respectively.

  • To make the ROSA26 genomic sequence compatible with the pBigT plasmid, pROSA26-1 was digested with XbaI, Klenow filled and a linker (PacI, SwaI, AscI) inserted.

  • The resulting plasmid was digested with SacI and PstI to remove the 5' MCS, which was replaced by a PacI linker.

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