Words similar to paci
Example sentences for: paci
How can you use “paci” in a sentence? Here are some example sentences to help you improve your vocabulary:
To make the ROSA26 genomic sequence compatible with the pBigT plasmid, pROSA26-1 was digested with XbaI, Klenow filled and a linker (PacI, SwaI, AscI) inserted.
A PacI site had been introduced into an EcoRI site in the exon encoding the second LIM domain of Isl1.
BigT was then digested with PacI and AscI to release the entire floxed neo-tpA and CFP assembly, and inserted into pROSA26PA digested with PacI and AscI.
The XbaI site used for insertion into the ROSA26 genomic locus [ 4] was replaced by a linker (PacI, SwaI, AscI), so that it could be digested with PacI and AscI, and receive the bigT sequence.
A cassette coating IRES Cre SV40 pA and pgk-neomycin was cloned into this PacI site to create a targeting construct with flanking 5' and 3' genomic DNA arms of 5 kb and 2 kb, respectively.