Words similar to pars-vn

Example sentences for: pars-vn

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To determine the distance permitted between the TATA box and the transcription initiation site in pARS-VN, we cloned different sized Alu targeting hooks (45/45 bp, 45/65 bp, 45/75 bp, 65/65 bp, 75/75 bp, and 115/75 bp) into vector Xho I- Cla I sites.
The hooks were PCR-amplified from the SV800 vector using specific primer pairs SV-ARS-F and SV-ARS-R (Table 2) and cloned into Xho I- Cla I sites of pARS-VN, yielding the vector pARS-TGAC.
The PCR fragments were inserted into a pRS313 polylinker [ 32 ] to generate the basic ARS -containing TAR vector designated as pARS-VN.