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Example sentences for: pars-tgac

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pARS-ALF contained two 40-bp fragments of alphoid DNA monomer that were PCR-amplified using the primer pair ALF-F and ALF-R from a 171-bp alphoid consensus sequence (X07685) [ 34 ] . None of the amplified targeting hooks contained an ATG codon that was out-of-frame in URA3 . Vectors pARS-TGAC and pARS-HPRT were linearized by Sal I, pARS-Alu by Sma I, and pARS-ALF by Mlu I, exposing the targeting sequences.
The hooks were PCR-amplified from the SV800 vector using specific primer pairs SV-ARS-F and SV-ARS-R (Table 2) and cloned into Xho I- Cla I sites of pARS-VN, yielding the vector pARS-TGAC.
The vector pARS-TGAC contained 60 bp of SV40 as a transgene-specific targeting hook and 50 bp of a common mouse repeat B1 as a second hook.