Words similar to pars-alf

Example sentences for: pars-alf

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The third vector, pARS-ALF, was designed for cloning human alphoid DNA arrays.
To apply the new selection strategy for isolation of large blocks of alphoid DNA, we created a TAR vector pARS-ALF with the structure
pARS-ALF contained two 40-bp fragments of alphoid DNA monomer that were PCR-amplified using the primer pair ALF-F and ALF-R from a 171-bp alphoid consensus sequence (X07685) [ 34 ] . None of the amplified targeting hooks contained an ATG codon that was out-of-frame in URA3 . Vectors pARS-TGAC and pARS-HPRT were linearized by Sal I, pARS-Alu by Sma I, and pARS-ALF by Mlu I, exposing the targeting sequences.