Words similar to pa-
Example sentences for: pa-
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We also examined duplicate clone filtering in a hybridization assay of two different samples, CaCO2 and PA-1 (Figure 4b).
To show how the concepts presented in this study can be applied to detect differentially regulated genes, we sought to identify sets of genes that are over- and under-expressed in ovary relative to colon by analyzing hybridization assays of CaCO2 (colon) and PA-1 (ovary) cell lines.
All 12 sequences in this region have high similarity to the derived consensus sequence GXGXXG(7X)A(SX)GXGXXG(4X)D(9X)R, which defines a nucleotide binding motif [ 16 25 ] . In addition, all sequences have five residues, or conservative substitutions in the case of Pa-MoeB, shown to participate in the adenylation reaction catalyzed by Ec-MoeB (Figure 3) [ 7 ] . The fact that such high similarities are retained in the ThiF coding region across three bacterial divisions suggests that this domain functions more or less autonomously.
A similar search using Pa-MoeB as the query yielded 146 sequences with a domain structure similar to MoeB, most of which were entered into the database as MoeB, ThiF, HesA, or ubiquitin-activating enzymes from archaea, bacteria, and eukaryota.
Ec-MoeB, Mt-MoeBR, Pa-MoeB, Pa-MoeBR, and Sc-MoeBR all contain two pairs of cysteine residues, each arranged in a CXXC motif and spaced 68-70 residues apart, like those in ThiF proteins (arrows in Figures 2cand 3).