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Example sentences for: paccmvplpa
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[ 30 ] and by us [ 31 ] . Human RGS3 cDNA [ 24 ] was restricted from the pRC/CMV vector with EcoRI, and then subcloned into the corresponding site of the pACCMVpLpa plasmid.
The procedure for preparation of the adenovirus was co-transfection of a human embryonic kidney cell line (HEK 293) with the pACCMVpLpa plasmid and another plasmid (pJM17) containing most of the viral genome [ 30 ] . A relatively rare recombination of the two plasmids occurs in the HEK 293 cells that reconstitutes the adenoviral genome.