Words similar to pbb
Example sentences for: pbb
How can you use “pbb” in a sentence? Here are some example sentences to help you improve your vocabulary:
5μl of ice-cold protein binding buffer (PBB) along with 10μl of GST-bead slurry corresponding to 3μg of fusion protein, 1μl of in vitro translated protein and 1.5μl of ligand or vehicle and/or peptides or vehicle.
The mix was incubated for two hours in the cold room with gentle agitation, the beads were pelleted by spinning briefly on a bench top Eppendorf centrifuge, washed four times with PBB containing no BSA, and the pellet was dried under vacuum for twenty minutes.
PBB was freshly prepared in 24 ml aliquots composed of 20 ml A-150 (20 mM Hepes, 150 mM KCl, 10 mM MgCl2 and 1% glycerol), and 2 ml each of phosphate buffered saline supplemented, respectively, with 1% Triton X-100 and 1% NP-40.