Words similar to polyt
Example sentences for: polyt
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Because first strand cDNA probes that are hybridized to such microarrays usually consist of poly(T)-containing molecules, which are produced by the reverse transcription of some tissue's poly(A) +RNA mixture, there is the potential for promiscuous polyA/polyT cross-hybridization between the first strand cDNA probes and the microarray DNA spots.
The T7-based IVT protocol for linear amplification of genomic DNA is shown in Figure 1. Since ChIP DNA is fragmented randomly and lacks a universal sequence for priming, we optimized a terminal transferase step to add polyT tails to the 3' ends of each DNA strand.
We suspect that the most important reason for the existence of a threshold signal is related to the formation of hybridization networks at these spots, which are also known as hyperpolymers [ 9 ] . If the polyT tail of a reverse-transcribed probe molecule binds promiscuously to the polyA in one of these spots, the remainder of the anchored probe molecule may hybridize to another labeled or unlabeled probe molecule in the hybridization solution, which in turn may hybridize to yet another probe molecule in the solution, etc., forming a network of hybridized probe molecules.
2) corresponds to the enteric smooth muscle form of gamma actin (Accession number T60048), which according to GenBank's annotation for that accession number is a possible reversed clone because polyT (or polyA in its complementary strand) was not found within it.
In fact, when microarrays were first being developed, investigators described situations in which their microarray hybridization data were dominated by this artifactual "polyA effect" (or its converse when polyA tails are in the labeled probe) [ 3 4 ] . To reduce the polyA effect, it is therefore common practice to add unlabeled polyA to the hybridization mixture to bind to the polyT segments of the hybridization probe, thus competing with the polyA segments in the microarray spots.
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