Words similar to polyt

Example sentences for: polyt

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The T7-based IVT protocol for linear amplification of genomic DNA is shown in Figure 1. Since ChIP DNA is fragmented randomly and lacks a universal sequence for priming, we optimized a terminal transferase step to add polyT tails to the 3' ends of each DNA strand.
In fact, when microarrays were first being developed, investigators described situations in which their microarray hybridization data were dominated by this artifactual "polyA effect" (or its converse when polyA tails are in the labeled probe) [ 3 4 ] . To reduce the polyA effect, it is therefore common practice to add unlabeled polyA to the hybridization mixture to bind to the polyT segments of the hybridization probe, thus competing with the polyA segments in the microarray spots.
We were surprised to find that there appear to be prominent artifacts related to promiscuous polyA/polyT hybridization only with very strongly expressed genes , which might be due to the formation of hybridization heteropolymers at the corresponding microarray spots.
PolyT tails were generated using terminal transferase (TdT) as follows.
Our protocol overcomes this obstacle by adding 3' polyT tails to the ends of the DNA fragments for use in priming much like mRNA polyA tails.