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Example sentences for: pon

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The stably transfected MCF7 line (MCF7-4EBP-1-5A) was treated with pon A and at various times cells were harvested for analysis of 4EBP-1 expression by Western blotting (Fig.
Conflicting results have been reported from studies on the stroke risk factor status of genes related to myocardial infarction or arterial disease, such as specific genotypes of the genes for angiotensin 1-converting enzyme ( ACE ) [ 23 24 25 26 ] or apolipoprotein [ 27 28 29 30 31 32 33 34 ] . Potential confounding factors include the effects of comorbidity and differential mortality rates [ 34 35 ] . Although the degree of stenosis of the cervical internal carotid arteries in symptomatic patients correlates with risk of ipsilateral stroke [ 36 ] , no clear conclusions can be drawn from attempts to relate carotid artery disease to specific genotypes (for example, of the paraoxonase gene PON, which is thought to protect low-density lipoprotein against oxidative modification [ 37 ] , or of the endothelial nitric oxide synthase gene [ 38 ] ).
The 4EBP-1-5A cDNA was placed under the control of a promoter that is responsive to the insect hormone ponasterone A (pon A), an ecdysone analog.
In the presence of pon A the ecdysone receptor recruits coactivators that lead to elevated expression.
Treatment of MCF7-pERV3 cells, which express the ecdysone receptor/VP16 fusion protein but not the inducible constitutively active 4EBP-1 (see Methods), with pon A had no effect on the expression of any of these proteins (Fig.