Words similar to pnpp

Example sentences for: pnpp

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Others have proposed that fatty acyl coenzyme A esters are potential physiologic regulators of PP5, since these compounds stimulate pNPP hydrolysis by PP5 in vitro at physiologically relevant concentrations and are approximately 10-fold more potent than their free fatty acid counterparts [ 21 ] . Although we confirmed this observation using PP5 and pNPP (data not shown), in our hands similar levels of AA and arachidoyl coenzyme A were required for half-maximal stimulation of 32P-MBP dephosphorylation in the case of both Ppt1p and PP5.
Phosphatase activity against pNPP or phosphohistone substrates was determined essentially as described previously.
max values (μmol Pi liberated / mg enzyme/ min) against these three substrates (pNPP, phosphoserine-histone, phosphotyrosine peptide) were, respectively: 12 +2, 8 +1, and 2 +0.
In the case of pNPP hydrolysis, others have reported both saturated and unsaturated fatty acyl coenzyme A esters stimulate PP5 activity, with concentrations required for half-maximal stimulation approximately 10-fold lower than those of their corresponding free fatty acids [ 21 ] . We therefore tested whether a saturated fatty acyl coenzyme A ester could also stimulate Ppt1p.
Although more work is required to examine this issue thoroughly, our results suggest that the large differences in potency between free fatty acids and fatty acyl coenzyme A esters seen using pNPP may not extend to all protein substrates.