Words similar to pmoles
Example sentences for: pmoles
How can you use “pmoles” in a sentence? Here are some example sentences to help you improve your vocabulary:
PCR reactions for deletion of residues 671-690, consisted of 10 nanograms pCR 2.1 TOPO/ HindIII-XhoI insert, 15 pmoles of each primer, 0.5 mM dNTPs, 1X cloned Pfu buffer (Stratagene) and 2.5 U cloned Pfu DNA polymerase (Stratagene).
The RT reaction products were mixed with 20 pmoles of primer SP-BF (5'-GACTAAGCCAGAGCAGAAGC-3') and 2 U of AmpliTaq DNA polymerase and then subjected to PCR which included 7 minutes at 94°C followed by 35 cycles of 94°C for 1 minute, 62°C for one minute, and 72°C for 1.5 minutes.
Analysis of the time course of relaxation with 6 pmoles of topoisomerase I or top67 reconstituted with ZD (Figure 2b) showed that negative supercoils were removed at a much slower rate by the reconstituted activity.
On the basis of these results 1.5 mM MgCl 2 , 10 pMoles primer and 50 ng of template DNA were considered to be optimal for generation of consistent RAPD profiles in mulberry and were, therefore, used in all the subsequent reactions with all the RAPD primers.
No change in B max was observed as a result of omitting the protease inhibitor (B max (+ protease inhibitor)= 3.1 ± 0.11 pmoles/mg protein vs B max (- protease inhibitor) = 3.2 ± 0.13 pmoles/mg protein.)