Example sentences for: pmoles

How can you use “pmoles” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • On the basis of these results 1.5 mM MgCl 2 , 10 pMoles primer and 50 ng of template DNA were considered to be optimal for generation of consistent RAPD profiles in mulberry and were, therefore, used in all the subsequent reactions with all the RAPD primers.

  • PCR reactions for deletion of residues 671-690, consisted of 10 nanograms pCR 2.1 TOPO/ HindIII-XhoI insert, 15 pmoles of each primer, 0.5 mM dNTPs, 1X cloned Pfu buffer (Stratagene) and 2.5 U cloned Pfu DNA polymerase (Stratagene).

  • The RT reaction products were mixed with 20 pmoles of primer SP-BF (5'-GACTAAGCCAGAGCAGAAGC-3') and 2 U of AmpliTaq DNA polymerase and then subjected to PCR which included 7 minutes at 94°C followed by 35 cycles of 94°C for 1 minute, 62°C for one minute, and 72°C for 1.5 minutes.

  • A single clone of transfected HEK cells stably expressing approximately 3 pmoles of μ opioid receptors per mg of protein, as measured by [ 3H]-DAMGO binding, was propagated and used as a model for characterization of the binding profiles of a set of opioid ligands at μ opioid receptors.

  • The cleavage assays were carried out with 1 pmole of 5' 32P-end labeled DNA substrate and 5-10 pmoles of topoisomerase I or Top67 in 10 μl of the buffer used for the gel mobility shift assay.


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