Words similar to pmoles
Example sentences for: pmoles
How can you use “pmoles” in a sentence? Here are some example sentences to help you improve your vocabulary:
The RT reaction products were mixed with 20 pmoles of primer SP-BF (5'-GACTAAGCCAGAGCAGAAGC-3') and 2 U of AmpliTaq DNA polymerase and then subjected to PCR which included 7 minutes at 94°C followed by 35 cycles of 94°C for 1 minute, 62°C for one minute, and 72°C for 1.5 minutes.
A single clone of transfected HEK cells stably expressing approximately 3 pmoles of μ opioid receptors per mg of protein, as measured by [ 3H]-DAMGO binding, was propagated and used as a model for characterization of the binding profiles of a set of opioid ligands at μ opioid receptors.
The final amplification reactions contained 10 mM TAPS (pH 8.8), 1.5 mM MgCl 2 , 50 mM KCI, 0.01% gelatin, 0.2 mM each dNTP, 10 pmoles primer, 0.6 U Taq DNA polymerase (Bangalore Genei, Bangalore, India) and 50 ng mulberry DNA template in a 25 μl reaction volume.
PCR reactions for deletion of residues 671-690, consisted of 10 nanograms pCR 2.1 TOPO/ HindIII-XhoI insert, 15 pmoles of each primer, 0.5 mM dNTPs, 1X cloned Pfu buffer (Stratagene) and 2.5 U cloned Pfu DNA polymerase (Stratagene).
This value agrees with published data for this compound [ 19 20 ] . B max values range from the 5.5 pmoles/mg protein observed for E148A to 0.30 pmoles/mg protein for the K153A mutation, indicating some variability in expression of the different receptors.