Words similar to pmole
Example sentences for: pmole
How can you use “pmole” in a sentence? Here are some example sentences to help you improve your vocabulary:
The proteins were mixed with the 1 pmole of the labeled DNA substrates in 10 μl of 20 mM Tris-HCl pH 8.0, 100 μg/ml BSA, 12% glycerol and 0.5 mM EDTA.
In brief each 50-ul reaction contained 50 ng of genomic DNA, 20 pmole of each primer, 1 unit of AmpliTaq Gold DNA polymerase, 5 ul of 10 X buffer, 2.5 ul of 2.5 mM of MgCl 2 & 2.5 mM of dNTPs.
The cleavage assays were carried out with 1 pmole of 5' 32P-end labeled DNA substrate and 5-10 pmoles of topoisomerase I or Top67 in 10 μl of the buffer used for the gel mobility shift assay.
These oligonucleotides (100 pmole) were annealed and extended with AMV reverse transcriptase (Amersham Pharmacia Biotech, Piscataway, NJ; 45 units) in AMV RT buffer (50 mM Tris-HCl, pH 8.3, 8 mM MgCl 2 , 50 mM NaCl, 1 mM DTT) and dNTPS (200 μM) for 30 minutes at 37°C.