Example sentences for: pmole

How can you use “pmole” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The cleavage assays were carried out with 1 pmole of 5' 32P-end labeled DNA substrate and 5-10 pmoles of topoisomerase I or Top67 in 10 μl of the buffer used for the gel mobility shift assay.

  • These oligonucleotides (100 pmole) were annealed and extended with AMV reverse transcriptase (Amersham Pharmacia Biotech, Piscataway, NJ; 45 units) in AMV RT buffer (50 mM Tris-HCl, pH 8.3, 8 mM MgCl 2 , 50 mM NaCl, 1 mM DTT) and dNTPS (200 μM) for 30 minutes at 37°C.

  • The proteins were mixed with the 1 pmole of the labeled DNA substrates in 10 μl of 20 mM Tris-HCl pH 8.0, 100 μg/ml BSA, 12% glycerol and 0.5 mM EDTA.

  • In brief each 50-ul reaction contained 50 ng of genomic DNA, 20 pmole of each primer, 1 unit of AmpliTaq Gold DNA polymerase, 5 ul of 10 X buffer, 2.5 ul of 2.5 mM of MgCl 2 & 2.5 mM of dNTPs.

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