Example sentences for: pmol

How can you use “pmol” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Individual primer-pair concentrations ranged from 0.31 to 3.30 pmol per reaction, based on the results of optimization runs.

  • The reactions were carried out in 50 μl using 5 pmol of each primer and cycled as follows: denaturation at 94°C for 45 s; annealing at 65°C for 45 s; and extension at 72°C for 45 s.

  • PCR amplification was performed on 1 μl of RT product (25 μM) incubated with 0.5 U of Taq DNA polymease (Promega) in a 25 μl reaction mixture containing 0.5 μM 10 mM dNTP, 1.5 μl of 25 mM MgCl, 2.5 μl of 10× Taq DNA polymerase buffer from Promega, 10 pmol of internal standard gene upstream and downstream primers to minimize variations in amplification efficiency between tubes.

  • One microgram of RNA was reverse transcribed to complementary (c)DNA using 200U Moloney murine leukemia virua (M-MLV) reverse transcriptase (GIBCO BRL Life Technologies, Grand Island, NY, USA), 1U RNAse inhibitor (5 Prime 3 Prime, Boulder, CO, USA) and 20 pmol oligo dT primer in a total volume of 20 μl.

  • 1 ± 1.7 nM, and a maximum binding capacity (B max ) of 2.8 pmol/mg of membrane protein.


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