Words similar to pmol
Example sentences for: pmol
How can you use “pmol” in a sentence? Here are some example sentences to help you improve your vocabulary:
Individual primer-pair concentrations ranged from 0.31 to 3.30 pmol per reaction, based on the results of optimization runs.
PCR amplification was performed on 1 μl of RT product (25 μM) incubated with 0.5 U of Taq DNA polymease (Promega) in a 25 μl reaction mixture containing 0.5 μM 10 mM dNTP, 1.5 μl of 25 mM MgCl, 2.5 μl of 10× Taq DNA polymerase buffer from Promega, 10 pmol of internal standard gene upstream and downstream primers to minimize variations in amplification efficiency between tubes.
Total incorporated Cy dye ranged from 300-600 pmol and the ratio of unlabeled to labeled nucleotides was typically between 25 and 50.
These reactions were carried out in 50 μ l using 5 pmol of each primer, and were cycled with a 9600 GeneAmp System (Perkin Elmer, Emeryville, CA, USA) as follows: denaturation at 94°C for 40 s; annealing at 65°C for 45 s; and extension at 72°C for 40 s.
The second standard sequencing reaction was primed from poly(A) tail using 1.6 pmol of a poly(T) primer anchored (PLYT 5'-TTTTTTTTTTTTTTTV-3' (V=A, C, or G)).
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