Words similar to pmol
Example sentences for: pmol
How can you use “pmol” in a sentence? Here are some example sentences to help you improve your vocabulary:
an appropriate nested primer is 5'- 32P-labeled as previously described [ 54 ] ; 5 μl of the P1+7A reaction or the step-in verification reaction is denatured and annealed with 1 pmol of the end-labeled display primer and 0.5 units of Taq polymerase (Qiagen) in 10 μl final volume of 1× "Taq salts" (Qiagen).
Active rates of taurocholate uptake were twice as high in the heterozygous cftr (+/-) mice (n = 23) compared to wild-type mice (n = 6) (average of all data points in the terminal ileum 109 ± 8 vs. 64 ± 13 cm pmol/mm 2; p < 0.05) and fourfold higher in the homozygous cftr (-/-) mice (n = 6) (212 ± 32 pmol/mm 2; p < 0.05 vs. wild-type).
PCR was performed in 50 μL reactions containing: template, 50 pmol each of primers F3 (5'-CATAACGCCAGCCCACCTACTG-3') and ETn1 (5'-CACGATTTGTGGGTAAAATAGGAG C-3'), 2.5 units Taq DNA polymerase, 0.2 mM dNTPs, 20 mM TrisHCl (pH 8.4), 50 mM KCl, and 1.5 mM MgCl.
15 pmol of nt 1932 to nt 1959 primer and 15 pmol of nt 3100 to 3081 primer were added and further cycled for 4 minutes at 95°C, then 30X of 2 minutes at 95°C, 1 minute at 60°C, 1 minute at 72°C and finally 10 minutes at 72°C.
Polymerase chain reaction (PCR) products (2 μ l) generated from either genomic DNA or cDNA were amplified using 5 pmol of nested sense V H family-specific FR3 primer and radiolabeled antisense nested J H consensus primer that had been end-labeled with γ 32-P (New England Nuclear, Beverly, MA, USA) using T4 polynucleotide kinase (Promega, Madison, WI, USA).
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