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Example sentences for: polyc

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Amplifications with each enzyme resulted in clones with variation in the length of the polyC tract (Figure 6C).
Variations of the length of the polyC tract of Cpn 043, Cpn 1054 and Cpn 1055 within C. pneumoniae AR39 were determined for 8-10 recombinant clones.
The variation of the length of the polyC tract within Cpn 043, 1054 and 1055 was determined through sequence analysis of purified amplification products and through sequencing of amplification products following cloning into plasmids.
Comparative genomic analysis and cloning expression showed that the length of the polyG tract of pmpG 10 varies between strains and within an isolate [ 12 13 ] . Furthermore, variation of the length of polyG has been demonstrated that it plays a role in the differential expression of PmpG 10 [ 12 ] . Variability in short nucleotide repeats generated via slipped strand mispairing are key elements in the generation of phenotypic diversity within many pathogenic microorganisms [ 18 ] . Further investigation will be required to determine if the expression of members of the Cpn 1054 gene family is affected by the observed variability in length of the polyC tract.
A second approach for examination of the possibility of PCR errors was to reamplify the polyC tract from a single plasmid template, and examine the sequence of the polyC tract directly in these amplification products.