Words similar to polya
Example sentences for: polya
How can you use “polya” in a sentence? Here are some example sentences to help you improve your vocabulary:
A widely used protocol, developed by Eberwine and colleagues, uses T7-based linear amplification [ 14 ] . In this procedure, double stranded cDNAs are synthesized from mRNA by reverse-transcription (priming off the 3' polyA tail) and second strand synthesis.
These arrays contain 9,589 probes (representing approximately 9200 unique accession numbers) designed to hybridize to human transcripts present in polyA+ RNA and approximately 386 control probes (designed to hybridize primarily to bacterial transcripts).
If the null hypothesis is that the muscle-probe hybridization values for spots with polyA values > 12000 have the same statistical distribution as spots with polyA values < 12000, then the probability that all eight of them have values greater than the median is (1/2) 8= 0.0039, and the probability that all eight of them have a value greater than 24000 is less than (52/567) 8which is less than 10 -8, because only 52 of the 567 detectable spots have values greater than 24000.
Thus, when the polyA effect is pronounced, the hybridization of the reverse transcription product made from any poly(A) +RNA mixture will be essentially the same, namely, the hybridization that would be obtained with an oligo(dT) probe alone.
Polymerization of ribonucleoside triphosphates (NTPs), which is central to a variety of crucial biological processes, including transcription, primer synthesis during DNA replication, addition of polyA tails to mRNA, addition of CCA to tRNA, uridylation in RNA editing, replication of RNA viruses, amplification of RNA in eukaryotic post-transcriptional gene silencing (PTGS), and oligoadenylate synthesis during interferon signaling, is catalyzed by a broad variety of distinct enzymes [ 1 2 3 4 ] . These polymerases belong to two major mechanistic categories, those that copy a nucleic acid template and those that are independent of a template.
Loading...