Words similar to polya
Example sentences for: polya
How can you use “polya” in a sentence? Here are some example sentences to help you improve your vocabulary:
To assess gene expression, total RNA isolated from normal testes, the cell lines, and tumor tissues, and polyA+ RNA of testis obtained from Clontech (Palo Alto, CA) was reverse transcribed using random primers and the Pro-STAR first strand RT-PCR kit (Stratagene, La Jolla, CA).
We were surprised to find that there appear to be prominent artifacts related to promiscuous polyA/polyT hybridization only with very strongly expressed genes , which might be due to the formation of hybridization heteropolymers at the corresponding microarray spots.
For expression studies, the vector sequences and all SV40 sequences except the SV40 3' UT and polyA addition site were removed by MluI digestion followed by purification on an agarose gel.
5 μg of polyA +RNA was converted to double stranded cDNA using the cDNA Synthesis Kit (Life Technologies, Gaithersburg, MD).
[ 4 ] , when this artifact is pronounced, the amount of hybridization at a particular microarray spot is an indication of the accessible polyA content of the DNA at the spot, rather than of the amount of the particular nucleic acid species in the hybridization solution that one intends to hybridize gene-specifically to that spot.