Example sentences for: polya

How can you use “polya” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Again, this contrasts with expression analysis where the mRNA 3' polyA tail provides a useful priming sequence for cDNA synthesis.

  • Polymerization of ribonucleoside triphosphates (NTPs), which is central to a variety of crucial biological processes, including transcription, primer synthesis during DNA replication, addition of polyA tails to mRNA, addition of CCA to tRNA, uridylation in RNA editing, replication of RNA viruses, amplification of RNA in eukaryotic post-transcriptional gene silencing (PTGS), and oligoadenylate synthesis during interferon signaling, is catalyzed by a broad variety of distinct enzymes [ 1 2 3 4 ] . These polymerases belong to two major mechanistic categories, those that copy a nucleic acid template and those that are independent of a template.

  • Resuspended probes were mixed with 4 μl of 5 × hybridization buffer (20 × SSX, 1%SDS), 1 μl human Cot-1 DNA (10 μg/μl), 1 μl polyA DNA (2 μg/μl) (Amersham) and 8 μl formamide (Sigma, St. Louis, MO), heated to 65°C for 2 min and then centrifuged briefly.

  • We therefore conclude that a polyA effect is not readily apparent in these data, presumably because we had added unlabeled polyA to the hybridization mixture as a blocking agent.

  • H i ' = H i - B i . However, looking along the vertical axis of the scatterplot, we were surprised to see that there are many spots at which H i is nearly zero, indicating that B i must also be nearly zero, even when there is a significant polyA signal.


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