Words similar to poly
Example sentences for: poly
How can you use “poly” in a sentence? Here are some example sentences to help you improve your vocabulary:
The most commonly used mechanism for RNA amplification is a T7 based linear amplification method first developed by Van Gelder, Eberwine and coworkers [ 19 20 21 ] . This method utilizes a synthetic oligo(dT) primer containing the phage T7 RNA polymerase promoter to prime synthesis of first strand cDNA by reverse transcription of the poly(A) +RNA component of total RNA.
Second, yCCR4 was shown to be a component, in association with yPOP2, of the major yeast cytoplasmic mRNA deadenylase complex, and to be required for efficient poly(A)-specific mRNA degradation [ 14 15 ] . yCCR4 might be a catalytic subunit of this complex, because of its nuclease-like domain.
The labeled cDNA was mixed with 1.5 μl of 10 μg/μl Cot-1 DNA, 1.5 μl of 8 μg/μl poly d(A), 1.5 μl of 4 μg/μl yeast tRNA, 4.5 μl of 20x SSC and 0.75 μl of 10% SDS, heated at 99°C for 2 minutes, and then incubated at 45°C for 15 minutes.
The idealized expression profile from the poly(A) +RNA virtual array is used as our unamplified "gold standard" for data analyses involving BC2.
The 3'-UTR is 123 nt in size, and contains a consensus polyadenylation signal (AAUAAA) 12 nucleotides from the poly(A) tail.