Example sentences for: poly-d

How can you use “poly-d” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR was performed on cDNA reverse transcribed from total RNA, extracted from lumbar DRGs, using poly-dT as a primer to obtain cDNA fragments (141 to 596 bp).

  • In short, double-stranded cDNA was synthesized from the total RNA using the SuperScript II kit from Invitrogen and the Affymetrix T7-(dT) 24 primer, which contains a T7 promoter attached to a poly-dT sequence 5'-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG-(dT) 24 -3'.

  • Poly-dT tailing with terminal transferase


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