Words similar to polyii

Example sentences for: polyii

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To generate pGaL4-hCCR4, a fragment corresponding to the entire hCCR4 coding sequence (with the exception of the initiation codon) was obtained upon digestion of the KIAA1194 cDNA (accession number AB033020, gift from the Kazusa Research Institute, putative start codon -Met 19-positioned by homology with mCCR4) by Sph I and Msc I (nt 423 to 2172 in KIAA1194), and was inserted in-frame into the pGaL4PolyII vector opened by Xho I, after Klenow treatment of both vector and insert.
To generate pGa14-m.nocturnin, a fragment from the m.nocturnin cDNA [ 21 ] corresponding to the entire coding sequence (with 9 amino acids upstream of the initiation codon) was excised from pGEX-3X upon Sma I and Dra I digestion, and was inserted into the pGaL4PolyII vector opened by Bam Hl, after Klenow treatment of both vector and insert.
The GAL4 fusion plasmids were obtained by subcloning the appropriate cDNAs into the pGaL4PolyII plasmid [ 24 ] , in-frame with the yeast GAL4 binding domain coding sequence.
Finally, the deleted open reading frame-containing fragment was excised using Sph I and Msc I, and inserted into the pGaL4PolyII vector opened by Xho I, after Klenow treatment of both vector and insert.