Example sentences for: polyacrylamide

How can you use “polyacrylamide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The reaction was stopped by the addition of 0.5 mol/l ethylenediaminetetra-acetic acid, and the labeled probe was purified on a 5% polyacrylamide gel.

  • Equal amounts of unboiled protein (60 μg) were loaded onto reducing 10% SDS-Tris-glycine polyacrylamide gels, transferred to nitrocellulose (Bio-Rad), and processed as described previously [ 51 ] , with some modifications.

  • 10 μl of the brush border membrane preparation was diluted in 90 μl of Laemmli buffer, boiled for 5 min and separated on a denaturing polyacrylamide gel (5 % stacking gel/10 % resolving gel) at 120 V over 2 hours [ 18 ] . The separated proteins were transferred onto nitrocellulose membrane using a Trans-blot instrument (Biorad, Hercules, CA) in glycine-methanol buffer following the manufacturer's suggestions.

  • For direct comparisons of protein expression between drug-treated and untreated cells, equal amounts of protein were loaded in adjacent lanes on a single polyacrylamide gel, separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions and transferred to nitrocellulose membranes by the semi-dry transfer method.

  • Hepatic RNA was extracted and quantified as we reported previously [ 10 ] . RNA was mixed with 32P-labeled c-myc, mdm2, p53, JunB, and β-actin probes, then co-precipitated, purified, and electrophoresed on 5% polyacrylamide gel.


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