Example sentences for: polyacrylamide

How can you use “polyacrylamide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Briefly, 10 μl of each sample were electrophoresed at 4°C and under non-denaturing conditions using a 10% polyacrylamide gel containing 0.05% gelatin.

  • Immune complexes were collected with Protein A agarose (Pierce), samples were electrophoresed on 15% polyacrylamide gels, and immunoblotting performed as described above.

  • 10 μl of the brush border membrane preparation was diluted in 90 μl of Laemmli buffer, boiled for 5 min and separated on a denaturing polyacrylamide gel (5 % stacking gel/10 % resolving gel) at 120 V over 2 hours [ 18 ] . The separated proteins were transferred onto nitrocellulose membrane using a Trans-blot instrument (Biorad, Hercules, CA) in glycine-methanol buffer following the manufacturer's suggestions.

  • The lysates were fractionated by polyacrylamide gel electrophoresis and then transferred to Immobilon-P membrane (Millipore Ltd., Nepean, ON).

  • SDS-PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis


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