Words similar to polyacrylamide
Example sentences for: polyacrylamide
How can you use “polyacrylamide” in a sentence? Here are some example sentences to help you improve your vocabulary:
Multiple antigens and antibodies have been patterned onto polystyrene using a desktop jet printer [ 19] and onto glass by covalent attachment to polyacrylamide gel pads [ 20] for parallel immunoassays.
10 μl of the brush border membrane preparation was diluted in 90 μl of Laemmli buffer, boiled for 5 min and separated on a denaturing polyacrylamide gel (5 % stacking gel/10 % resolving gel) at 120 V over 2 hours [ 18 ] . The separated proteins were transferred onto nitrocellulose membrane using a Trans-blot instrument (Biorad, Hercules, CA) in glycine-methanol buffer following the manufacturer's suggestions.
SDS-PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis
The radioactively labeled PCR products were separated on 6% denaturing polyacrylamide sequencing gels, and the alleles were visualized by autoradiography [ 6 ] . For the fluorescently labeled products, α- 32P-dCTP was omitted, fluorescent primers were used, and the products were analyzed in an ABI PrismTM 377 DNA Sequencer (Perkin Elmer) following the manufacturer's specifications.
Equal amounts of unboiled protein (60 μg) were loaded onto reducing 10% SDS-Tris-glycine polyacrylamide gels, transferred to nitrocellulose (Bio-Rad), and processed as described previously [ 51 ] , with some modifications.