Words similar to ligation

Example sentences for: ligation

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Similarly, OCP ligation/ERCA can be used to accurately genotype PCR products in a biallelic format, where the probes for both alleles are present in a single reaction [ 3 ] . The OCP ligation/ERCA method is based on ligation dependent circularization of allele specific DNA probes followed by exponential rolling circle amplification of the circularized probes [ 4 5 ] . ERCA probes have also been variously described as 'padlock probes' [ 6 7 ] or 'circularizable probes' [ 4 ] . Although OCP ligation/ERCA can be used to genotype PCR products, it also provides a means for directly genotyping genomic DNA without requiring an initial amplification of the genomic DNA locus.
The ligation products were amplified with an adapter-specific primer and either the probe-specific forward primer RATPase6F or SP-BF (for the T7 adapter ligation) or the probe-specific reverse primer RATPase6R or SP-BR (for the SP6 adapter ligation).
DNA denaturation, annealing and ligation reactions were carried out in an Eppendorf Master Cycler (Eppendorf Scientific, Germany).
As expected, OCP ligation/ERCA results were influenced by the amount of genomic DNA used in the assay.
Specificity of the OCP to its target sequence is achieved by the complementarity of the two ends of the OCP to the target and the requirement of these ends to be adjacent for ligation.