Words similar to ligation

Example sentences for: ligation

How can you use “ligation” in a sentence? Here are some example sentences to help you improve your vocabulary:

This linker encoded in frame the amino acids MSHHHHHHSM, with the last residue corresponding to the first methionine encoded in rec12 +exon 1. Trimolecular ligation was used to create pREP2- rec12 +, pREP42- rec12 +, and pREP82- rec12 +clones encoding Rec12 protein with 6X histidine tags.
The PCR fragments were ligated to the plasmid DNA and the ligation products were used to transform strain DH5α.
Effect of CD81 ligation on long-term T cell activation
Standard methods of restriction enzyme digestion and ligation of selected fragments were applied to generate the various expression constructs.
Single nucleotide discrimination is achieved at the ligation step by the use of the thermostable DNA ligase, Ampligase, which has a high affinity for a perfectly matched substrate at the 3' end of a DNA molecule [ 17 ] . We were able to enhance allele discrimination at the ligation step by designing the OCP such that the 5' complementary region is firmly hybridized to the target sequence whereas the 3' region is in equilibrium with its target at the ligation temperature [ 12 ] . This would result in increased specificity since the correctly matched OCP will have a greater chance to act as substrate for the ligase.