Words similar to ligation
Example sentences for: ligation
How can you use “ligation” in a sentence? Here are some example sentences to help you improve your vocabulary:
FII ligation reaction conditions
Reactions conditions were the same as for kinetics of the ligation reaction above except for the reaction temperatures indicated.
Kinetics of the ligation reaction
Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.
In general, current methods require pre-amplification of genomic DNA, typically by Polymerase Chain Reaction (PCR), followed by SNP genotyping with an allele discrimination method, such as DNA cleavage, ligation, single base extension or hybridization [ 5 ] . Current methods are limited either by expense, inaccuracy, consumption of sample DNA, or lack of scalability.