Example sentences for: ligation

How can you use “ligation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • FII ligation reaction conditions

  • Reactions conditions were the same as for kinetics of the ligation reaction above except for the reaction temperatures indicated.

  • Kinetics of the ligation reaction

  • Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.

  • In general, current methods require pre-amplification of genomic DNA, typically by Polymerase Chain Reaction (PCR), followed by SNP genotyping with an allele discrimination method, such as DNA cleavage, ligation, single base extension or hybridization [ 5 ] . Current methods are limited either by expense, inaccuracy, consumption of sample DNA, or lack of scalability.


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