Words similar to ligation
Example sentences for: ligation
How can you use “ligation” in a sentence? Here are some example sentences to help you improve your vocabulary:
Those who didn't advocate voluntary tubal ligation (I hope that was what you were advocating) thought religion was in order.
Single nucleotide discrimination is achieved at the ligation step by the use of the thermostable DNA ligase, Ampligase, which has a high affinity for a perfectly matched substrate at the 3' end of a DNA molecule [ 17 ] . We were able to enhance allele discrimination at the ligation step by designing the OCP such that the 5' complementary region is firmly hybridized to the target sequence whereas the 3' region is in equilibrium with its target at the ligation temperature [ 12 ] . This would result in increased specificity since the correctly matched OCP will have a greater chance to act as substrate for the ligase.
Thus, direct ligation of CD81 on T cells was sufficient to induce costimulation of T cell activation.
The DNA samples were digested with the restriction endonuclease Alu I before being used as template in the ligation reaction.
DNA denaturation, annealing and ligation reactions were carried out in an Eppendorf Master Cycler (Eppendorf Scientific, Germany).