Example sentences for: ligation

How can you use “ligation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • OCP ligation was performed with Ampligase for 20 min at 60°C, approximately 15°C above the Tm for the OCP 3' arm, in order to maximize specificity of ligation.

  • 2. Upon annealing, sticky ends were generated that were suitable for ligation into the pET/T7p/T7 autogene construct cleaved with BglII and XbaI.

  • Standard methods of restriction enzyme digestion and ligation of selected fragments were applied to generate the various expression constructs.

  • ERCA is capable of 10 12-fold signal amplification [ 6 ] . In order to investigate the efficiency of the ERCA step, OCP was circularized by ligation on a synthetic oligonucleotide target, and gel-purified to remove any linear DNA.

  • Thus, direct ligation of CD81 on T cells was sufficient to induce costimulation of T cell activation.


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