Example sentences for: ligation

How can you use “ligation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • DNA was ethanol precipitated from the ligation reaction and resuspended in water; 200 ng DNA was electroporated into Electromax DH5α cells.

  • This removes the 5' phosphate from incomplete mRNA fragments and from non-mRNAs eliminating their ability to participate in subsequent ligation reactions.

  • OCP ligation/ ERCA probes can be multiplexed.

  • DNA purification and plasmid ligation were performed with commercial kits following the manufacturer's instructions: DNA purification from the agarose-gel with the QUIAquick kit (Quiagen, Hilden, Germany), and bacterial cloning with the TA-cloning kit (Invitrogen, Leek, The Netherlands).

  • In reactions where the OCP matches the SNP, an estimated 100-30,000 OCPs are circularized by ligation depending on the amount of input DNA target.


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