Example sentences for: ligating

How can you use “ligating” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Autogene libraries were constructed by first generating vectors containing promoter mutations, and then ligating polymerase gene libraries into these vectors.

  • Plasmid pBPhin2 was created by ligating the Bgl II-digested Rv2233-Rv2235 fragment into the Bam HI site of pBPhin.

  • The plasmid pMP8NEBΔLacZ was generated by ligating an 8 kb Xba1 fragment from pMP8 into the Xba1 site of pNEB193 (New England Biolabs) from which LacZ sequences had been removed as a NarI/EcoRI fragment [ 15 ] . This

  • Plasmid pBPhin1 was constructed by ligating a Bam HI-digested PCR fragment containing the Rv0365c gene plus 24 bp located upstream of it to Bam HI-digested pBPhin.

  • 7, lacked ~50 codons corresponding to the 5' end of the human EP2 receptor and which were present in the rabbit genomic sequence, a full-length sequence of the rabbit EP 2 was constructed by ligating a SacII/XhoI cDNA fragment (nucleotide 340-1655) from the cDNA clone 18A with the upstream Hind III/SacII fragment (nucleotide 1-340) of the rabbit genomic clone.


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