Words similar to ligating

Example sentences for: ligating

How can you use “ligating” in a sentence? Here are some example sentences to help you improve your vocabulary:

The plasmid pMP8NEBΔLacZ was generated by ligating an 8 kb Xba1 fragment from pMP8 into the Xba1 site of pNEB193 (New England Biolabs) from which LacZ sequences had been removed as a NarI/EcoRI fragment [ 15 ] . This
A MCS containing the restriction sites for XhoI, ApaI, SacII, NotI, SacI, EcoRV, and KpnI was synthesized and inserted downstream of the second loxP site by digesting the DNA with XhoI and KpnI and ligating in the MCS, which had been synthesized to have compatible cohesive ends.
α i2 (1-10)-GFP was constructed by annealing the complementary oligonucleotides 5'-ctagcaccatgggctgcaccgtgtcggccgaggacaag-3' and 5'-ccggcttgtcctcggacacggtgcagcccatggtg-3' (corresponding to amino acids 1-10 of α i2 ) and ligating into AgeI-NheI digested pEGFP-N1.
Plasmid pBPhin2 was created by ligating the Bgl II-digested Rv2233-Rv2235 fragment into the Bam HI site of pBPhin.
Autogene libraries were constructed by first generating vectors containing promoter mutations, and then ligating polymerase gene libraries into these vectors.