Example sentences for: ligating

How can you use “ligating” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Autogene libraries were constructed by first generating vectors containing promoter mutations, and then ligating polymerase gene libraries into these vectors.

  • 7, lacked ~50 codons corresponding to the 5' end of the human EP2 receptor and which were present in the rabbit genomic sequence, a full-length sequence of the rabbit EP 2 was constructed by ligating a SacII/XhoI cDNA fragment (nucleotide 340-1655) from the cDNA clone 18A with the upstream Hind III/SacII fragment (nucleotide 1-340) of the rabbit genomic clone.

  • A MCS containing the restriction sites for XhoI, ApaI, SacII, NotI, SacI, EcoRV, and KpnI was synthesized and inserted downstream of the second loxP site by digesting the DNA with XhoI and KpnI and ligating in the MCS, which had been synthesized to have compatible cohesive ends.

  • The plasmid pMP8NEBΔLacZ was generated by ligating an 8 kb Xba1 fragment from pMP8 into the Xba1 site of pNEB193 (New England Biolabs) from which LacZ sequences had been removed as a NarI/EcoRI fragment [ 15 ] . This

  • Plasmid pBPhin1 was constructed by ligating a Bam HI-digested PCR fragment containing the Rv0365c gene plus 24 bp located upstream of it to Bam HI-digested pBPhin.


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