Words similar to ligation-mediated
Example sentences for: ligation-mediated
How can you use “ligation-mediated” in a sentence? Here are some example sentences to help you improve your vocabulary:
LM-PCR, ligation-mediated polymerase chain reaction; MAC, macronucleus; MIC, micronucleus; TASs, telomere addition sites; CR-R, CR-L, Common Region-Right, -Left.
The first protocol uses a ligation-mediated PCR strategy (LM-PCR) in which ChIP DNA is ligated to short DNA linkers, and then PCR amplified using the linker sequence for priming [ 4 ] . The second uses a random amplification strategy (R-PCR) in which template DNA is first copied using a specialized primer with degenerate 3' sequence and known 5' sequence, and then PCR amplified using the known 5' sequence for priming [ 5 8 9 ] . In both cases, the PCR steps yield quantities of nucleic acid sufficient for microarray analysis.