Example sentences for: ligated

How can you use “ligated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Each filament was applied perpendicularly to the plantar surface of the ligated paw of rats kept in suspended wire-mesh cages.

  • Each PCR product was ligated to adapters that contained an SP6 promoter sequence (SP6 adapter) in one reaction or to adapters with a T7 promoter sequence (T7 adapter) in another reaction according to the Lig'nScribe™ (Ambion, Austin, TX) protocol.

  • The first protocol uses a ligation-mediated PCR strategy (LM-PCR) in which ChIP DNA is ligated to short DNA linkers, and then PCR amplified using the linker sequence for priming [ 4 ] . The second uses a random amplification strategy (R-PCR) in which template DNA is first copied using a specialized primer with degenerate 3' sequence and known 5' sequence, and then PCR amplified using the known 5' sequence for priming [ 5 8 9 ] . In both cases, the PCR steps yield quantities of nucleic acid sufficient for microarray analysis.

  • 10-15% of the OCP matching the SNP were ligated in 15 sec (Fig.

  • The PCR product was digested with Eco RI and Bam HI and ligated into the Eco RI and Bam HI restriction sites of pGADA424 to produce construct pGAD424/LTBP-3 EGF#1-8.


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