Example sentences for: ligated

How can you use “ligated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • A NotI (blunt) 3.6 kb lacZ fragment from pCMV-βgal (Clontech) was ligated into this SalI (blunt) site to generate a targeting vector that could be used to introduce lacZ into the "marked" Hnf3 α locus.

  • The PCR-based enrichment of differentially expressed sequences depends on the number of tester molecules with adaptors ligated to their ends.

  • To generate a library of recombinant plasmids, 200 ng of the ligated sample were electroporated into Electromax DH5α cells.

  • After confirmation of the sequence, the mutated pL1PUC19 plasmid was digested with Nde I and Hind III, and the 900 bp, mutated L1 gene was gel purified and ligated into pET26b to create the mutant overexpression plasmids.

  • The L5 and L6 spinal nerves were tightly ligated by a square knot with 6-0 silk thread.


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