Words similar to ligated
Example sentences for: ligated
How can you use “ligated” in a sentence? Here are some example sentences to help you improve your vocabulary:
10-15% of the OCP matching the SNP were ligated in 15 sec (Fig.
The gel-purified fragment was ligated with Cla I linker and then cut with Cla I and Hin cII to produce the ~400-bp fragment containing the enhancer [ 7].
Digested fragments were ligated with corresponding adapters and then amplified using one-base selective primers ( Hind III-A and Mse I-T) according to standard AFLP protocol [ 22 ] . Three biotinylated SSR probes, (AT) 15 , (GT) 15 , (GA) 15 , which were reported being rich in other plant species, were separately hybridized with amplified AFLPs.
The over-expression plasmid for L1, pUB5832, was digested with Nde I and Hind III, and the resulting ca. 900 bp piece was gel purified and ligated using T4 ligase into pUC19, which was also digested with Nde I and Hind III, to yield the cloning plasmid pL1PUC19.
The fusion gene was then cut from the 6His-HA-PP1α-pcDNA3 plasmid and ligated into pTEP4m, which contains a tetracycline response element for inducible expression [ 25 ] , and a hygromycin resistance gene for selection in eukaryotic cells (see Figure 1).