Words similar to ligated
Example sentences for: ligated
How can you use “ligated” in a sentence? Here are some example sentences to help you improve your vocabulary:
The 18-mer duplex DNA encoding the insertion peptide (Ile-Asn-Asn-Glu-Thr-Ile) was ligated with Hpa1 digested pKK-RT66 in between codon 135 and 136.
Isothermal exponential amplification produces a fluorescent signal from ligated OCP using nanomolar amounts of probe.
1) The ble gene was PCR amplified from pPICZalphaC as a 375-bp fragment using primers Ble-N and Ble-C; the amplicon was blunt-ended and ligated into the blunt-cloning site of pT7Blue to generate pT7Blue-bleA.
The PCR product was digested with Xba I and Mro I and ligated into pBS-KS +-NFATp that had been digested with Xba I and Mro I. pVL1392-HAX-NFATp(1-722) was made by subcloning a Nde I - Msc I fragment from pBS-KS +-NFATp(NdeI) into the Nde I and Sma I sites of pVL1392-HAX.
DNA fragments containing AQP1 DNA were liberated from pSKAC after restriction and ligated into adenovirus as described previously [ 27 ] . Human embryonic kidney cells (293 cells) were transfected with ligation mixture and individual viruses were isolated from cell lysates by two consecutive rounds of plaque purification using an agar overlay as described previously [ 27 ] . Individual viruses were amplified in 293 cells and purified over cesium step gradient.
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