Words similar to ligated
Example sentences for: ligated
How can you use “ligated” in a sentence? Here are some example sentences to help you improve your vocabulary:
The genetic mobility assay was performed essentially as described in Guo et al ., 2000 (Figure 4) [ 25 ] . In brief, the donor plasmids (carrying the mutant or wild-type introns) were each co-transformed with a recipient plasmid pBRR3, carrying a promoterless tet R gene downstream of the target site (which are the ligated exons; positions -30/+15).
For mismatched OCP, less than 1% was ligated after 6 hours (Fig.
The cDNA was digested with Eco RI and Xho I, size separated (retaining molecules approximately 200 bp or larger), ligated into HybriZAP™ vector arms, and packaged into phage heads for amplification.
During the second step, the 3' hydroxyl of the 5' exon attacks the phosphate at the 3' splice site, yielding a ligated mRNA and a lariat intron.
A NotI (blunt) 3.6 kb lacZ fragment from pCMV-βgal (Clontech) was ligated into this SalI (blunt) site to generate a targeting vector that could be used to introduce lacZ into the "marked" Hnf3 α locus.
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