Example sentences for: ligated

How can you use “ligated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The exponential amplification of the ligated probe sequence generates double stranded DNA products that are unit lengths of the probe [ 4 ] . Electrophoretic separation on an agarose gel generates a ladder of DNA bands as seen in Figure 3. Typically, the products ranged in size from 80 bases to 1.2 kb, with most products less than 500 bases long.

  • The fusion gene was then cut from the 6His-HA-PP1α-pcDNA3 plasmid and ligated into pTEP4m, which contains a tetracycline response element for inducible expression [ 25 ] , and a hygromycin resistance gene for selection in eukaryotic cells (see Figure 1).

  • The same amount of Taq I fragments for each clone was ligated to the CT and TT adapters at 16°C overnight.

  • The cassette was then ligated into the channel cDNA cut with the same two restriction enzymes.

  • The genetic mobility assay was performed essentially as described in Guo et al ., 2000 (Figure 4) [ 25 ] . In brief, the donor plasmids (carrying the mutant or wild-type introns) were each co-transformed with a recipient plasmid pBRR3, carrying a promoterless tet R gene downstream of the target site (which are the ligated exons; positions -30/+15).


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