Words similar to ligated
Example sentences for: ligated
How can you use “ligated” in a sentence? Here are some example sentences to help you improve your vocabulary:
The exponential amplification of the ligated probe sequence generates double stranded DNA products that are unit lengths of the probe [ 4 ] . Electrophoretic separation on an agarose gel generates a ladder of DNA bands as seen in Figure 3. Typically, the products ranged in size from 80 bases to 1.2 kb, with most products less than 500 bases long.
The fusion gene was then cut from the 6His-HA-PP1α-pcDNA3 plasmid and ligated into pTEP4m, which contains a tetracycline response element for inducible expression [ 25 ] , and a hygromycin resistance gene for selection in eukaryotic cells (see Figure 1).
The same amount of Taq I fragments for each clone was ligated to the CT and TT adapters at 16°C overnight.
The cassette was then ligated into the channel cDNA cut with the same two restriction enzymes.
The genetic mobility assay was performed essentially as described in Guo et al ., 2000 (Figure 4) [ 25 ] . In brief, the donor plasmids (carrying the mutant or wild-type introns) were each co-transformed with a recipient plasmid pBRR3, carrying a promoterless tet R gene downstream of the target site (which are the ligated exons; positions -30/+15).