Words similar to ligase
Example sentences for: ligase
How can you use “ligase” in a sentence? Here are some example sentences to help you improve your vocabulary:
The Xylella Acl exhibited greater identity with phenylacetate-CoA ligase of E. coli than with anthranilate-CoA ligase of A. evansii , but a given substrate specificity within homology groups often can be associated with different subgroupings [ 25, 34].
The digestion products were treated with T4 DNA ligase and transformed into E. coli XL 1-Blue.
The F-box-containing proteins of the SCF complex constitute a family of E3 ligases, key components of the ubiquitin-proteasome pathway [ 67 ] , as are many RING finger proteins [ 68 ] . The RING finger motif is thought to mediate protein-protein interactions and E3 ligase complex assembly.
For example, Herschlag and co-workers have previously shown that a nucleobase could complement an abasic site within the hammerhead ribozyme, restoring cleavage activity [ 1 ] . Similarly, Porta and Lizardi engineered a hammerhead ribozyme that assumed an inactive secondary structure; upon the addition of an oligonucleotide that disrupted the engineered secondary structure, the ribozyme was activated [ 2 ] . More recently, it has previously been shown that many selected nucleic acid binding species (aptamers) undergo conformational changes upon ligand binding [ 3 4 5 6 ] In addition, when aptamers are conjoined to ribozymes, the ligand-induced conformational changes in the aptamers frequently modulate the structures and hence the activities of the ribozymes [ 7 8 9 ] For example, binding of theophylline to the anti-theophylline aptamer [ 10 ] modulates the conformation of residue [ 11 12 ] and the overall stability of the aptamer [ 10 13 ] . Appending the anti-theophylline aptamer to the hammerhead ribozyme allowed cleavage activity to be modulated by 110-fold by theophylline [ 13 ] . Similarly, appending the anti-theophylline aptamer to a small ribozyme ligase (L1) generated an aptazyme that was activated 1,600-fold by saturating theophylline concentrations [ 14 ] .
Although ACC was not regulated at the transcriptional level, cytoplasmic citrate (generated from the Acly reaction) is an allosteric activator of ACC, so less cytoplasmic citrate would lead to less activation of ACC [ 45 ] . Transcript levels of hepatic acetyl CoA synthetase 1 AMP forming (acetate:CoA ligase AMP forming; Acas1) were consistently down regulated by LC-PUFA (-2.
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