Words similar to ligase
Example sentences for: ligase
How can you use “ligase” in a sentence? Here are some example sentences to help you improve your vocabulary:
Because of its role in mediating the degradation of HY5, HYH, and possibly other transcription factors in Arabidopsis [ 4 5 ] and the presence of a RING finger domain and a WD-40 domain, AtCOP1 has been proposed to function as a RING-finger type ubiquitination E3 ligase, by recruiting E2 through its RING-finger domain and binding to the substrates through the WD-40 repeats [ 4 43 ] . This hypothesis is also supported by the findings about other negative regulators of plant photomorphogenesis.
It is plausible to suggest that the inhibition in E2F-1 protein levels by SAHA was either at the transcription level or induction of the ubiquitin-protein ligase responsible for E2F-1 degradation, but not E2F-4, and this resulted in blocked DNA synthesis.
The PCR reaction was treated with 10 U of DpnI (Stratagene) and recircularized with T4 DNA ligase (Stratagene).
Molecular modeling has shown that this domain contains two zinc finger-like motifs connected through linking C 3 HC 4 regions [ 18 ] . Naturally occurring splice variants of the gene suggest at least two transcription initiation points above and below the coding region for the RING domain [ 19 ] . Truncation studies have shown that the RING domain may function in direct protein binding of ER-α, ATF1, and BARD1, a ubiquitin ligase [ 20 21 22 ] . While zinc RING domains are common motifs in several protein families such as oncoproteins and regulatory proteins, the actual function of the domain differs among these proteins.
The Taq I fragments of HL60 cDNA were ligated with the CT adapter at 16°C overnight with 3 μl (9 units) of T4 ligase (Promega, Madison, WI) while the Taq I fragments of HL60/TLK286 cDNA were ligated with the TT adapter.
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