Words similar to ligase
Example sentences for: ligase
How can you use “ligase” in a sentence? Here are some example sentences to help you improve your vocabulary:
Second strand cDNA synthesis was finished using DNA polymerase I and T4 DNA ligase.
The correlation coefficient between amplified and unamplified sample and the yield of aRNA amplified without DNA ligase are high enough to suggest that DNA ligase activity is not necessary for RNA amplification in microarray analysis.
Nucleic acid-based assays, such as the polymerase chain reaction (PCR), the ligase chain reaction (LCR), nucleic acid sequence-based amplification (NASBA), branched chain DNA (bDNA) and sequence analysis itself, rely on the efficient hybridization of oligonucleotides to the targeted sequence.
The first step in the genotyping assay involves SNP interrogation by ligation of the OCP using the thermostable ligase, Ampligase™, which is reported to have excellent discrimination between a matched and mismatched 3' terminal nucleotide [ 17 ] . In order to directly investigate the efficiency and specificity of the ligation step, allele discrimination of OCP ligation was analyzed using 5' 32P-labeled OCP.
Studies in budding yeast identified SCF Cdc4p, an E3 ubiquitin ligase complex that mediates the ubiquitylation of the CDK inhibitor Sic1p [ 3 4 ] . SCF Cdc4pconsists of at least four proteins: the cullin Cdc53p, the RING domain protein Hrt1p/Rbx1p/Roc1p, the adapter protein Skp1p, and Cdc4p (reviewed in [ 1 ] ). Cdc4p contains two sequence motifs, which are conserved in a wide variety of so-called F-box proteins: C-terminal WD-repeats that are involved in binding the substrate Sic1p in a phosphorylation-dependent manner, and a central F-box [ 5 ] that interacts with Skp1p [ 6 7 ] . Cdc53p in turn binds to Skp1p and Hrt1p/Rbx1p/Roc1p, which bridges Cdc53p with the ubiquitin-conjugating enzyme Cdc34p/Ubc3p [ 6 8 9 10 11 12 ] . In vitro reconstitution demonstrated that SCF Cdc4p, Cdc34p, E1, ubiquitin, and ATP are sufficient to mediate Sic1p polyubiquitylation [ 6 7 ] .
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