Words similar to ars
Example sentences for: ars
How can you use “ars” in a sentence? Here are some example sentences to help you improve your vocabulary:
The mutants in group C (Δ10, Δ13 and Δ14) generated the smallest colonies (20-38 % of the area of those generated by the control, which was intact extended ars3002 ). The mutants in group B (Δ7, Δ11, Δ12, Δ16-Δ20) produced larger colonies (50-79 % of control).
The intron-less pce1 +gene was amplified from plasmid pl32-PCE1 [ 12 ] . The PCR products were digested with Xho I and Bam HI and then inserted into the S. pombe expression vector pREP41X ( LEU2 ars1 +) [ 19 ] . The inserts were sequenced to exclude the acquisition of unwanted mutations during the amplification and cloning steps.
It eliminates the limitation of the previously described TAR cloning protocols by eliminating the need for an ARS sequence in the region to be cloned.
We consider the enhancer to be a functional part of both ars3002 and ars3004 . We found that the enhancer could not be substituted by a DNA fragment of the same size and base composition but different nucleotide sequence [ 7].
All analyses were performed as described previously [ 7, 10], except that transformation plates were analyzed after 4-5 days, since the growth rates of colonies transformed with enhancer-containing plasmids are faster than those with core ars3002 alone (which was used in the previous experiment [ 10]).