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Example sentences for: arpe-

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H 2 O 2 induced cytotoxicity in RPE cells appears to be affected by the density of cells in cultures [ 29 ] . To study this phenomenon, ARPE-19 cells plated in 96-well plates with densities of 10,000, 20,000 or 40,000 cells/well were subjected to H 2 O 2 treatment.
ARPE-19 is a spontaneous occurring, non-transformed RPE cell line which expresses RPE specific markers (such as CRALBP and RPE65) and exhibits morphological polarization and tight junctions similar to native RPE [ 25 26 ] .
Cells were plated in 96-well plates (human primary RPE: 10,000 cells/well, ARPE-19: 15,000-20,000 cells/well, unless otherwise stated) overnight, fed with serum-free medium or treated with testing agents (prepared in serum-free medium) the next day followed by H 2 O 2 treatment for a day, then the viability from each treatment was determined by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay [ 27 ] . Culture medium was removed after treatment and then 100 μl MTT solution (100 μg/ml prepared in DMEM) was added to each well.
ARPE-19 cells were treated with various concentrations (0.
Since ERK activation appeared to be an early event associated with H 2 O 2 induced cytotoxicity, experiments were performed to determine if inhibitors of ERK activation could affect the cytotoxicity caused by H 2 O 2 . ARPE-19 cells were pretreated with 10 μM PD98059 (a MEK inhibitor which functions upstream of ERK [ 33 ] ) followed by H 2 O 2 challenge.