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Example sentences for: arsh
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The ars operon consists of a group of genes coding for a transmembrane pump and an arsenate reductase ( arsC ). The operon includes a regulatory gene ( arsR ) and a gene coding for an arsenite-specific pump ( arsB ) as well as arsC [ 13 ] . Arsenite is pumped directly out of the cell by the arsB protein; however arsenate must first be reduced to arsenite by the cytoplasmic arsenate reductase coded from arsC . Some bacteria also possess other ars genes: arsA produces an arsenite-stimulated ATPase [ 14 ] that results in more efficient arsenite extrusion; arsD encodes for a regulatory protein that controls the upper level of ars expression [ 15 ] ; arsH has been identified but has an uncertain function [ 16 ] . The ars operon was initially recognized in plamids of Staphylococcus aureus and S. xylosus [ 17 18 ] but has subsequently been found in other microorganisms (e.g.
SAT-pADH1-CEN6-ARSH4-HIS3-URA3-SAT
The TAR vector pARS-SV211-URA was constructed by insertion of the S. cerevisiae URA3 gene between the targeting hooks of pVC-SV211 [ 7 ] and cloning of ARSH4 into the polylinker.
As in the first approach, the TAR vector contained a yeast centromere ( CEN6 ), a yeast ARS element ( ARSH4 ), a positive-selectable marker ( HIS3 ), and a negative-selectable marker ( URA3 ). In this approach, however, the targeting hooks are placed between the promoter and the open reading frame of the URA3 gene.
All those vectors were created with the Bluescript-based yeast- E. coli shuttle vector pRS313 (HIS3-CEN6-ARSH4) [ 32 ] . The cassette containing the URA3 gene driven by the Shizosaccharomyces pombe ADH1 promoter was derived from the pBL94 plasmid [ 19 ] . In the original cassette, the ADH1 promoter and URA3 were separated by a 35-bp adaptor containing an out-of-frame ATG initiation codon.
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