Words similar to arsh
Example sentences for: arsh
How can you use “arsh” in a sentence? Here are some example sentences to help you improve your vocabulary:
As in the first approach, the TAR vector contained a yeast centromere ( CEN6 ), a yeast ARS element ( ARSH4 ), a positive-selectable marker ( HIS3 ), and a negative-selectable marker ( URA3 ). In this approach, however, the targeting hooks are placed between the promoter and the open reading frame of the URA3 gene.
SAT-pADH1-CEN6-ARSH4-HIS3-URA3-SAT
The ars operon consists of a group of genes coding for a transmembrane pump and an arsenate reductase ( arsC ). The operon includes a regulatory gene ( arsR ) and a gene coding for an arsenite-specific pump ( arsB ) as well as arsC [ 13 ] . Arsenite is pumped directly out of the cell by the arsB protein; however arsenate must first be reduced to arsenite by the cytoplasmic arsenate reductase coded from arsC . Some bacteria also possess other ars genes: arsA produces an arsenite-stimulated ATPase [ 14 ] that results in more efficient arsenite extrusion; arsD encodes for a regulatory protein that controls the upper level of ars expression [ 15 ] ; arsH has been identified but has an uncertain function [ 16 ] . The ars operon was initially recognized in plamids of Staphylococcus aureus and S. xylosus [ 17 18 ] but has subsequently been found in other microorganisms (e.g.
The TAR vector pARS-SV211-URA was constructed by insertion of the S. cerevisiae URA3 gene between the targeting hooks of pVC-SV211 [ 7 ] and cloning of ARSH4 into the polylinker.
All those vectors were created with the Bluescript-based yeast- E. coli shuttle vector pRS313 (HIS3-CEN6-ARSH4) [ 32 ] . The cassette containing the URA3 gene driven by the Shizosaccharomyces pombe ADH1 promoter was derived from the pBL94 plasmid [ 19 ] . In the original cassette, the ADH1 promoter and URA3 were separated by a 35-bp adaptor containing an out-of-frame ATG initiation codon.
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