Words similar to amplitaq

Example sentences for: amplitaq

How can you use “amplitaq” in a sentence? Here are some example sentences to help you improve your vocabulary:

The cycling parameters were as follows: (1) 50°C × 30 min, reverse transcription single-strand synthesis (with one, both or neither primer); (2) 95°C × 15 min, activate AmpliTaq polymerase, inactivate RT enzymes; (3) 4°C, add missing primers; (4) 94°C × 30 sec, commence PCR cycling; (5) 56°C × 30 sec; (6) 72°C × 30 sec; (7) go to step 4 (30 cycles in total); (8) 72°C × 10 min.
PCR (32 cycles) was performed on an ABI 877 Catalyst robotic workstation (5 μl reactions - 5 ng of genomic DNA, 2.5 mM MgCl 2 , 0.2 mM dNTPs [Pharmacia, Piscataway, NJ, USA], 0.2 U Amplitaq Gold DNA polymerase [Perkin-Elmer, Foster City, CA, USA] in 1 × PCR Buffer II [Perkin-Elmer]).
Automated sequencing reactions were performed using ABI PRISM Dye Terminator (or dRhodomine for PLYT reactions) Cycle Sequencing Ready Reaction Kits with AmpliTaq DNA polymerase, FS, according to the manufacturer's directions or as described for octamer-primed sequencing reactions [ 42, 43].
The RT reaction products were mixed with 20 pmoles of primer SP-BF (5'-GACTAAGCCAGAGCAGAAGC-3') and 2 U of AmpliTaq DNA polymerase and then subjected to PCR which included 7 minutes at 94°C followed by 35 cycles of 94°C for 1 minute, 62°C for one minute, and 72°C for 1.5 minutes.
DNA sequencing was carried out by cycle sequencing using the PRISM Big Dye Terminator sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Division of PE/ABI).