Example sentences for: amplitaq

How can you use “amplitaq” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In brief each 50-ul reaction contained 50 ng of genomic DNA, 20 pmole of each primer, 1 unit of AmpliTaq Gold DNA polymerase, 5 ul of 10 X buffer, 2.5 ul of 2.5 mM of MgCl 2 & 2.5 mM of dNTPs.

  • 2 μM), 0.25 μl AmpliTaq (Applied Biosystems, Foster City, California) and 10 μl of RT-PCR product in a final volume of 50 μl.

  • 2 ; Perkin Elmer, Norwalk, CT, USA), and Amplitaq DNA polymerase (2.

  • The cycling parameters were as follows: (1) 50°C × 30 min, reverse transcription single-strand synthesis (with one, both or neither primer); (2) 95°C × 15 min, activate AmpliTaq polymerase, inactivate RT enzymes; (3) 4°C, add missing primers; (4) 94°C × 30 sec, commence PCR cycling; (5) 56°C × 30 sec; (6) 72°C × 30 sec; (7) go to step 4 (30 cycles in total); (8) 72°C × 10 min.

  • DNA sequencing was carried out by cycle sequencing using the PRISM Big Dye Terminator sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Division of PE/ABI).


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