Example sentences for: amplitaq

How can you use “amplitaq” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The PCR reaction mixture consisted of 1 × buffer A (Perkin-Elmer), 3 mM MgCl 2 , 200 μM dNTPs, 90 nM of each adenovirus primer, 100 nM of each 18S rDNA primer, 200 nM of each probe, 0.6 U AmpliTaq Gold polymerase (Perkin-Elmer) and 5 μl of total DNA sample.

  • The structure of all reporter constructs were confirmed by DNA sequencing (Amplitaq sequencing kit, Perkin-Elmer).

  • Automated sequencing reactions were performed using ABI PRISM Dye Terminator (or dRhodomine for PLYT reactions) Cycle Sequencing Ready Reaction Kits with AmpliTaq DNA polymerase, FS, according to the manufacturer's directions or as described for octamer-primed sequencing reactions [ 42, 43].

  • Fifty μl PCR reactions contained 1X Perkin Elmer PCR buffer with 1.5 mM MgCl 2 , 0.8 mM each dNTP, 1.25 U AmpliTaq DNA polymerase (Perkin Elmer), and 45 μM of each primer.

  • 2 , 0.4 μM TNF-α primer or COX-2 primer, 1:19 ratio of 18S competimers/primers, 0.1 mM each dNTP, 0.2 μl α [ 32P] dCTP (10 mCi/ml; > 3000 Ci/mmol) and 0.025 units of Amplitaq (Perkin Elmer Inc., Norwalk, CT, USA).


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