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Example sentences for: amplitaq

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DNA sequencing was carried out by cycle sequencing using the PRISM Big Dye Terminator sequencing kit and AmpliTaq DNA polymerase (Perkin-Elmer, Division of PE/ABI).
PCR amplification was performed, with final concentration of the digest equivalent to approximately 100 ng of the DNA, 1 × PCR bufferII (Perkin-Elmer, Foster City, CA, USA), 2.5 mmol/l MgCl 2 , and amplitaq gold 1.25 units.
The sequences and necessary concentrations in the PCR reaction are outlined in Table 2. The PCR mixture included 0.625 U AmpliTaq Gold DNA polymerase in the 2× SYBR Green Master Mix (PE Biosystems), the required concentration of specific forward and reverse primers, 10 ng of cDNA template and 0.25 U AmpErase UNG (PE Biosystems) in a 25 μl reaction volume.
In brief each 50-ul reaction contained 50 ng of genomic DNA, 20 pmole of each primer, 1 unit of AmpliTaq Gold DNA polymerase, 5 ul of 10 X buffer, 2.5 ul of 2.5 mM of MgCl 2 & 2.5 mM of dNTPs.
The cycling conditions were as follows: denaturation at 95°C/12 min to activate the amplitaq gold, followed by 35 cycles of denaturation at 95°C/45 s, annealing at 60°C/30 s and extension at 72°C/30 s.