Example sentences for: amplitaq

How can you use “amplitaq” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • PCR (32 cycles) was performed on an ABI 877 Catalyst robotic workstation (5 μl reactions - 5 ng of genomic DNA, 2.5 mM MgCl 2 , 0.2 mM dNTPs [Pharmacia, Piscataway, NJ, USA], 0.2 U Amplitaq Gold DNA polymerase [Perkin-Elmer, Foster City, CA, USA] in 1 × PCR Buffer II [Perkin-Elmer]).

  • 3), 5 mM KCl, 1.5 mM MgCl 2 , 200 μM of each dNTP, and 2.5 units of Amplitaq (Perkin-Elmer/Cetus).

  • 2 ; Perkin Elmer, Norwalk, CT, USA), and Amplitaq DNA polymerase (2.

  • Automated sequencing reactions were performed using ABI PRISM Dye Terminator (or dRhodomine for PLYT reactions) Cycle Sequencing Ready Reaction Kits with AmpliTaq DNA polymerase, FS, according to the manufacturer's directions or as described for octamer-primed sequencing reactions [ 42, 43].

  • In brief each 50-ul reaction contained 50 ng of genomic DNA, 20 pmole of each primer, 1 unit of AmpliTaq Gold DNA polymerase, 5 ul of 10 X buffer, 2.5 ul of 2.5 mM of MgCl 2 & 2.5 mM of dNTPs.

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