Words similar to amplitaq
Example sentences for: amplitaq
How can you use “amplitaq” in a sentence? Here are some example sentences to help you improve your vocabulary:
Each 25 μl PCR mix contained 7.875 μl Sybr ®Green Master Mix (1X Sybr ®Green Buffer, 3 mM MgCl 2 , 200 nM dNTP blend, 0.6275 U Amplitaq Gold ®, 0.25 U AmpErase ®UNG), 200-400 nM forward and reverse primers and approximately 5 ng template.
2 ; Perkin Elmer, Norwalk, CT, USA), and Amplitaq DNA polymerase (2.
3), 5 mM KCl, 1.5 mM MgCl 2 , 200 μM of each dNTP, and 2.5 units of Amplitaq (Perkin-Elmer/Cetus).
PCR (32 cycles) was performed on an ABI 877 Catalyst robotic workstation (5 μl reactions - 5 ng of genomic DNA, 2.5 mM MgCl 2 , 0.2 mM dNTPs [Pharmacia, Piscataway, NJ, USA], 0.2 U Amplitaq Gold DNA polymerase [Perkin-Elmer, Foster City, CA, USA] in 1 × PCR Buffer II [Perkin-Elmer]).
The structure of all reporter constructs were confirmed by DNA sequencing (Amplitaq sequencing kit, Perkin-Elmer).