Example sentences for: amplitaq

How can you use “amplitaq” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The sequences and necessary concentrations in the PCR reaction are outlined in Table 2. The PCR mixture included 0.625 U AmpliTaq Gold DNA polymerase in the 2× SYBR Green Master Mix (PE Biosystems), the required concentration of specific forward and reverse primers, 10 ng of cDNA template and 0.25 U AmpErase UNG (PE Biosystems) in a 25 μl reaction volume.

  • The reactions were carried out in the Universal PCR master mix, containing MgCl 2 , dNTP and DNA polymerase AmpliTaq Gold™ (Perkin-Elmer) in a total volume of 50 μl.

  • 5 μCi of α-[ 35S] dATP (1270 Ci/mmole), and 1 U of AmpliTaq DNA polymerase (Perkin-Elmer, Foster City, CA).

  • PCR (32 cycles) was performed on an ABI 877 Catalyst robotic workstation (5 μl reactions - 5 ng of genomic DNA, 2.5 mM MgCl 2 , 0.2 mM dNTPs [Pharmacia, Piscataway, NJ, USA], 0.2 U Amplitaq Gold DNA polymerase [Perkin-Elmer, Foster City, CA, USA] in 1 × PCR Buffer II [Perkin-Elmer]).

  • 2 μM), 0.25 μl AmpliTaq (Applied Biosystems, Foster City, California) and 10 μl of RT-PCR product in a final volume of 50 μl.

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