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Example sentences for: amplifluors

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We describe here a simple, scalable assay for SNP genotyping directly from human genomic DNA that uses a 96-well plate format and fluorescent primers called Amplifluors™ [ 13 14 15 12 16 ] . Ten different SNPs have been characterized, each for DNA samples from 192 different individuals, using this reporter assay system.
The two Amplifluors are labeled with a differently colored fluorophore: fluorescein (FAM) and tetrachloro-6-carboxyfluorescein (TET) and contain an internal nonfluorescent quencher DABCYL.
By comparison, Amplifluors typically required 60-120 minutes to generate signal [ 8 ] . The substantial difference in time to generate signal may be due to the nature of Amplifluor design.