Words similar to ampliscribe
Example sentences for: ampliscribe
How can you use “ampliscribe” in a sentence? Here are some example sentences to help you improve your vocabulary:
The Epicentre AmpliScribe T7 High Yield Transcription kit was used to produce unlabeled cRNA by in vitro transcription with unbiotinylated NTPs.
The PCR product (2 μg in 50 μl) was added to an in vitro transcription reaction containing Ampliscribe T7 RNA polymerase (Epicentre), RNase inhibitor (GIBCO BRL, Rockville, MD; 5 units), low Mg 2+buffer (30 mM Tris-HCl, pH 8, 7.5 mM DTT, 4.5 mM MgCl 2 , 1.5 mM spermidine), UTP (1.
Biotinylated cRNA was prepared using the standard Affymetrix protocol [ 8 ] except that the Epicentre AmpliScribe™ T7 polymerase (Epicentre, Madison, WI) was substituted for the ENZO T7 polymerase (BioArray, High Yield RNA Transcript Labeling Kit, ENZO Diagnostics, Inc., Farmingdale, NY) for 2 of the treated and 2 of the untreated pooled samples.
Polyadenylated control RNA was prepared from these clones by in-vitro transcription of linearized plasmids using the AmpliScribe transcription kit (Epicenter Technologies, Madison, WI) following the manufacturer's instructions.
This lack of difference led us to select the Epicentre Ampliscribe™ T7 High Yield Kit for the extra in vitro transcription step to decrease expense.