Example sentences for: ampliscribe

How can you use “ampliscribe” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The PCR product (2 μg in 50 μl) was added to an in vitro transcription reaction containing Ampliscribe T7 RNA polymerase (Epicentre), RNase inhibitor (GIBCO BRL, Rockville, MD; 5 units), low Mg 2+buffer (30 mM Tris-HCl, pH 8, 7.5 mM DTT, 4.5 mM MgCl 2 , 1.5 mM spermidine), UTP (1.

  • This lack of difference led us to select the Epicentre Ampliscribe™ T7 High Yield Kit for the extra in vitro transcription step to decrease expense.

  • Biotinylated cRNA was prepared using the standard Affymetrix protocol [ 8 ] except that the Epicentre AmpliScribe™ T7 polymerase (Epicentre, Madison, WI) was substituted for the ENZO T7 polymerase (BioArray, High Yield RNA Transcript Labeling Kit, ENZO Diagnostics, Inc., Farmingdale, NY) for 2 of the treated and 2 of the untreated pooled samples.

  • The Epicentre AmpliScribe T7 High Yield Transcription kit was used to produce unlabeled cRNA by in vitro transcription with unbiotinylated NTPs.

  • Polyadenylated control RNA was prepared from these clones by in-vitro transcription of linearized plasmids using the AmpliScribe transcription kit (Epicenter Technologies, Madison, WI) following the manufacturer's instructions.


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