Words similar to ampligase

Example sentences for: ampligase

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0.5 nM wt OCP and 0.5 nM mut OCP (final concentration); 0.5 U ampligase.
50 ng to 1 μg of either genomic DNA or WGA genomic sample was mixed with OCP (typically 0.5 nM final concentration) and 0.5 unit of Ampligase (Epicentre Technologies, Madison, WI) in 1x Ampligase buffer (Epicentre), for a total volume of 10 μl.
Reactions were started by adding Ampligase at 0.2 units/μl final concentration in a reaction volume of 0.1 ml.
Single nucleotide discrimination is achieved at the ligation step by the use of the thermostable DNA ligase, Ampligase, which has a high affinity for a perfectly matched substrate at the 3' end of a DNA molecule [ 17 ] . We were able to enhance allele discrimination at the ligation step by designing the OCP such that the 5' complementary region is firmly hybridized to the target sequence whereas the 3' region is in equilibrium with its target at the ligation temperature [ 12 ] . This would result in increased specificity since the correctly matched OCP will have a greater chance to act as substrate for the ligase.
Reactions were started by adding Ampligase at 0.2 units/μl final concentration and a reaction volume of 0.1 ml.