Words similar to ampligase

Example sentences for: ampligase

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Reactions were started by adding Ampligase at 0.2 units/μl final concentration and a reaction volume of 0.1 ml.
All ligation reactions were in 1x Ampligase Reaction Buffer supplied by Epicentre Technologies: 20 mM Tris-HCl (pH8.
Single nucleotide discrimination is achieved at the ligation step by the use of the thermostable DNA ligase, Ampligase, which has a high affinity for a perfectly matched substrate at the 3' end of a DNA molecule [ 17 ] . We were able to enhance allele discrimination at the ligation step by designing the OCP such that the 5' complementary region is firmly hybridized to the target sequence whereas the 3' region is in equilibrium with its target at the ligation temperature [ 12 ] . This would result in increased specificity since the correctly matched OCP will have a greater chance to act as substrate for the ligase.
OCP ligation was performed with Ampligase for 20 min at 60°C, approximately 15°C above the Tm for the OCP 3' arm, in order to maximize specificity of ligation.
0.1 nM wt OCP and 1.2 nM mut OCP (final concentration); 0.5 U ampligase.