Example sentences for: ampligase

How can you use “ampligase” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • OCP ligation was performed with Ampligase for 20 min at 60°C, approximately 15°C above the Tm for the OCP 3' arm, in order to maximize specificity of ligation.

  • 0.1 nM wt OCP and 1.2 nM mut OCP (final concentration); 0.5 U ampligase.

  • 0.5 nM wt OCP and 0.5 nM mut OCP (final concentration); 0.5 U ampligase.

  • All components except Ampligase were pre-warmed to the reaction temperature (40, 47, 55, or 63°C) for 20 min to allow OCP annealing to come to equilibrium.

  • The reactions were set up in 96-well MicroAmp Optical plates (Perkin Elmer) in a 10 μl reaction volume containing 1 U Ampligase (Epicentre Technologies), 20 mM Tris-HCl (pH 8.3), 25 mM KCl, 10 mM MgCl 2 , 0.5 mM NAD, and 0.01% Triton ®X-100.


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