Words similar to ampligase
Example sentences for: ampligase
How can you use “ampligase” in a sentence? Here are some example sentences to help you improve your vocabulary:
Reactions were started by adding Ampligase at 0.2 units/μl final concentration in a reaction volume of 0.1 ml.
All components except Ampligase were pre-warmed to the reaction temperature (40, 47, 55, or 63°C) for 20 min to allow OCP annealing to come to equilibrium.
0.1 nM wt OCP and 1.2 nM mut OCP (final concentration); 0.5 U ampligase.
The reactions were set up in 96-well MicroAmp Optical plates (Perkin Elmer) in a 10 μl reaction volume containing 1 U Ampligase (Epicentre Technologies), 20 mM Tris-HCl (pH 8.3), 25 mM KCl, 10 mM MgCl 2 , 0.5 mM NAD, and 0.01% Triton ®X-100.
All ligation reactions were in 1x Ampligase Reaction Buffer supplied by Epicentre Technologies: 20 mM Tris-HCl (pH8.
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