Example sentences for: amplified

How can you use “amplified” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The data can only be evaluated quantitatively between samples amplified with the same primer set.

  • The entire NF1 -coding region from two normal non-affected individuals was amplified in 5 overlapping segments by RT-PCR according to Heim [ 17 ] starting from fresh blood leukocytes.

  • These mRNAs could be amplified en masse, re-cloned, and re-transformed into E. coli.

  • The HCMV DNA was detected using HCMV1 (5' cct agt gtg gat gac cta cgg gcc a) and HCMV2 (5' cag aca cag tgt cct ccc gct cct c) primers producing 249 bp long amplicon and the DNA of HHV6 was amplified with specific primer pair HP0 (5' ccg caa tcg aat cca cct agc gg) and HP4 (5' gtg aga acg gat tcg aac agt gct g) yielding 440 bp product [ 23 24 ] . All amplifications were carried out with 20 pmol of each primer in 2 mM solution of MgCl 2 , 2 U of Tag Special DNA polymerase (Biovendor, Czech Republic), 0.3 mM of each dNTPs, 10× reaction buffer and 1 μg of isolated DNA according to the following conditions: 96°C for 4 min, (94°C for 10 sec, 58°C for 10 sec, 72°C for 20 sec) 36 times, and final extension at 72°C for 2 min.

  • The principles of this method are detailed in references [ 17 ] and [ 18 ] . Briefly, approximately 300 bp fragments of each candidate gene, with the SNP site in the middle, will be amplified by automated PCR procedure.


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