Words similar to amplified
Example sentences for: amplified
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The HCMV DNA was detected using HCMV1 (5' cct agt gtg gat gac cta cgg gcc a) and HCMV2 (5' cag aca cag tgt cct ccc gct cct c) primers producing 249 bp long amplicon and the DNA of HHV6 was amplified with specific primer pair HP0 (5' ccg caa tcg aat cca cct agc gg) and HP4 (5' gtg aga acg gat tcg aac agt gct g) yielding 440 bp product [ 23 24 ] . All amplifications were carried out with 20 pmol of each primer in 2 mM solution of MgCl 2 , 2 U of Tag Special DNA polymerase (Biovendor, Czech Republic), 0.3 mM of each dNTPs, 10× reaction buffer and 1 μg of isolated DNA according to the following conditions: 96°C for 4 min, (94°C for 10 sec, 58°C for 10 sec, 72°C for 20 sec) 36 times, and final extension at 72°C for 2 min.
The principles of this method are detailed in references [ 17 ] and [ 18 ] . Briefly, approximately 300 bp fragments of each candidate gene, with the SNP site in the middle, will be amplified by automated PCR procedure.
Briefly, amplified and purified prokaryotic control vectors were linearized at the 5' end using Xho I and purified by gel electrophoresis.
In this context, the autoimmune phenotypes resulting from the combination of the 129 and B6 genomes may might therefore provide a primed background upon which the effects of deficiency in the target gene can be amplified.
The Amplified Differential Gene Expression (ADGE) technique was designed to quadratically magnify the ratios of gene expression [ 6 ] . Briefly, the control and tester cDNA's are digested with Taq I restriction enzyme, then ligated with the CT and TT adapters, respectively.
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