Words similar to amplification
Example sentences for: amplification
How can you use “amplification” in a sentence? Here are some example sentences to help you improve your vocabulary:
Amplification reactions were performed in a total volume of 40 μl and contained 2 μl eluted phage, 40 ng of each primer (FADI 5'-CACTACAATGGATGATG-3' and RADI 5'-CTTGCGGGGTTTTTCAG-3'), 0.001% Tween 20 (Sigma), 2.5 U Taq DNA polymerase (Promega), 1x Taq polymerase buffer (Promega), 1.56 mM MgCl 2 (Promega), and 0.25 mM of each dNTP (USB).
The resulting cDNA was used as template for PCR amplification using the primer pairs (Operon Technologies, Alameda, CA, USA) listed in Table 1. As a loading control parallel PCR reactions were carried out using a primer pair for human GAPDH (see Table 1).
Such broad-range PCR strategies have been used to identify and characterize several known and previously uncharacterized bacteria [ 5 6 ] and viruses [ 7 8 ] . In order to maximize the utility of these effective pathogen nucleic acid amplification systems, amplification needs to be coupled with rapid, sensitive, and specific detection.
Specific amplification was determined relative to constant region control PCR.
The primers used for amplification of methylated and unmethylated promoters of genes RARB , TIMP3 , CDKN2A , p14 ARF , MGMT , DAPK , CDH1 , GSTP1 , APC promoter 1A, RB1 , MLH1 , TP73 , BRCA1 , FHIT , and HIC1 have been described previously http://pathology2.jhu.edu/pancreas/prim0425.htm#MSP; [ 32 47 48 49 ] . Methylated (MF and MR) and unmethylated (UF and UR) primers for RASSF1A promoter were designed according to Herman et al [ 46 ] and as described earlier [ 50 ] . PCR products were run on 2% agarose gels and visualized after ethidium bromide staining.
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