Words similar to amplicon
Example sentences for: amplicon
How can you use “amplicon” in a sentence? Here are some example sentences to help you improve your vocabulary:
Next, we compared oligonucleotide-derived ratios with those obtained from PCR amplicon probes (Figure 4c).
First, the PCR amplicon for H +-ATPase also contains the same 20 contiguous nucleotides (Figure 9).
A correlation coefficient of r = 0.80 ( p < 0.05) and a slope close to unity were obtained, indicating that unmodified oligonucleotide and PCR amplicon probes gave comparable expression ratios.
PCR assays and amplicon sequencing
The HCMV DNA was detected using HCMV1 (5' cct agt gtg gat gac cta cgg gcc a) and HCMV2 (5' cag aca cag tgt cct ccc gct cct c) primers producing 249 bp long amplicon and the DNA of HHV6 was amplified with specific primer pair HP0 (5' ccg caa tcg aat cca cct agc gg) and HP4 (5' gtg aga acg gat tcg aac agt gct g) yielding 440 bp product [ 23 24 ] . All amplifications were carried out with 20 pmol of each primer in 2 mM solution of MgCl 2 , 2 U of Tag Special DNA polymerase (Biovendor, Czech Republic), 0.3 mM of each dNTPs, 10× reaction buffer and 1 μg of isolated DNA according to the following conditions: 96°C for 4 min, (94°C for 10 sec, 58°C for 10 sec, 72°C for 20 sec) 36 times, and final extension at 72°C for 2 min.
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