Words similar to amphotropic
Example sentences for: amphotropic
How can you use “amphotropic” in a sentence? Here are some example sentences to help you improve your vocabulary:
A transinfection technique was used to rapidly establish a polyclonal amphotropic producer line of moderate to high titer.
Adherent cells were transduced with an amphotropic retrovirus, DFG-hIL-1β-neo, which encodes the mature form of human IL-1β fused to the leader sequence of human parathyroid hormone to enable efficient secretion, and neomycin phosphotransferase [ 21 ] . Retroviral transductants were positively selected in complete DMEM containing Geneticin at 0.5 mg/ml.
Supernatant, generated from transfected GPE86 cells [ 31], was then used to infect the amphotropic packaging cell line PA317 [ 31] in the presence of 4 μg/ml polybrene (Abbott Laboratories, Abbott Park, IL, USA).
Cells were grown to approximately 75% confluence and incubated in the presence of 8 μg/ml polybrene with 2 ml supernatant containing amphotropic retrovirus DFG-IRAP-zeo rcontaining the human IL-1Ra and Streptoalloteichus hindustanus bleomycin-resistance ( Sh ble ; zeo r) genes.
Cherry Hill, NJ, USA), and incubated with amphotropic PA317 packaging cells for 24 hours.