Example sentences for: amphotropic

How can you use “amphotropic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Isolation of amphotropic producer cells

  • Supernatant, generated from transfected GPE86 cells [ 31], was then used to infect the amphotropic packaging cell line PA317 [ 31] in the presence of 4 μg/ml polybrene (Abbott Laboratories, Abbott Park, IL, USA).

  • Adherent cells were transduced with an amphotropic retrovirus, DFG-hIL-1β-neo, which encodes the mature form of human IL-1β fused to the leader sequence of human parathyroid hormone to enable efficient secretion, and neomycin phosphotransferase [ 21 ] . Retroviral transductants were positively selected in complete DMEM containing Geneticin at 0.5 mg/ml.

  • A transinfection technique was used to rapidly establish a polyclonal amphotropic producer line of moderate to high titer.

  • The murine fibroblast NIH 3T3 cell line (CCL 92) and the amphotropic retroviral packaging cell line PA317 (CRL 9078) were obtained from the American Type Culture Collection (Rockville, MD, USA).


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