Example sentences for: amphotropic

How can you use “amphotropic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were grown to approximately 75% confluence and incubated in the presence of 8 μg/ml polybrene with 2 ml supernatant containing amphotropic retrovirus DFG-IRAP-zeo rcontaining the human IL-1Ra and Streptoalloteichus hindustanus bleomycin-resistance ( Sh ble ; zeo r) genes.

  • Cherry Hill, NJ, USA), and incubated with amphotropic PA317 packaging cells for 24 hours.

  • The murine fibroblast NIH 3T3 cell line (CCL 92) and the amphotropic retroviral packaging cell line PA317 (CRL 9078) were obtained from the American Type Culture Collection (Rockville, MD, USA).

  • Isolation of amphotropic producer cells

  • Adherent cells were transduced with an amphotropic retrovirus, DFG-hIL-1β-neo, which encodes the mature form of human IL-1β fused to the leader sequence of human parathyroid hormone to enable efficient secretion, and neomycin phosphotransferase [ 21 ] . Retroviral transductants were positively selected in complete DMEM containing Geneticin at 0.5 mg/ml.


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