Words similar to amphotropic
Example sentences for: amphotropic
How can you use “amphotropic” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were grown to approximately 75% confluence and incubated in the presence of 8 μg/ml polybrene with 2 ml supernatant containing amphotropic retrovirus DFG-IRAP-zeo rcontaining the human IL-1Ra and Streptoalloteichus hindustanus bleomycin-resistance ( Sh ble ; zeo r) genes.
Isolation of amphotropic producer cells
A transinfection technique was used to rapidly establish a polyclonal amphotropic producer line of moderate to high titer.
The murine fibroblast NIH 3T3 cell line (CCL 92) and the amphotropic retroviral packaging cell line PA317 (CRL 9078) were obtained from the American Type Culture Collection (Rockville, MD, USA).
Murine fibroblast NIH 3T3 cells, amphotropic PA317 packaging cells, and Phoenix E ecotropic packaging cells were cultured in Dulbecco's modified Eagle's medium (DMEM)-high glucose (GIBCO-BRL, Grand island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (GIBCO-BRL, Grand island, NY, USA), 100 U/ml penicillin, 100 μg/ml streptomycin, and 200 mM L-glutamine.