Example sentences for: amphotropic

How can you use “amphotropic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The murine fibroblast NIH 3T3 cell line (CCL 92) and the amphotropic retroviral packaging cell line PA317 (CRL 9078) were obtained from the American Type Culture Collection (Rockville, MD, USA).

  • A transinfection technique was used to rapidly establish a polyclonal amphotropic producer line of moderate to high titer.

  • Supernatant, generated from transfected GPE86 cells [ 31], was then used to infect the amphotropic packaging cell line PA317 [ 31] in the presence of 4 μg/ml polybrene (Abbott Laboratories, Abbott Park, IL, USA).

  • Murine fibroblast NIH 3T3 cells, amphotropic PA317 packaging cells, and Phoenix E ecotropic packaging cells were cultured in Dulbecco's modified Eagle's medium (DMEM)-high glucose (GIBCO-BRL, Grand island, NY, USA) supplemented with 10% heat-inactivated fetal bovine serum (GIBCO-BRL, Grand island, NY, USA), 100 U/ml penicillin, 100 μg/ml streptomycin, and 200 mM L-glutamine.

  • Cherry Hill, NJ, USA), and incubated with amphotropic PA317 packaging cells for 24 hours.


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