Words similar to amphotericin
Example sentences for: amphotericin
How can you use “amphotericin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Oocytes were transferred into tubes containing Hepes-buffered TCM-199 (Gibco BRL, Grand Island, NY) supplemented with 0.57 mM cysteine, 10 ng/ml epidermal growth factor (Sigma), 100 IU/ml penicillin, 100 μg/ml streptomycin, 0.25 ng/ml amphotericin, 0.01 IU/ml porcine FSH (Sioux Biochemicals, Sioux Center, IA), 0.01 IU/ml porcine LH (Sioux Biochemicals) and 0.1% (w:v) polyvinyl alcohol (maturation medium) [ 50], and shipped to the laboratory overnight (20-24 h) at 38.
According to available reports, treatment in Latin America usually consists of one of the azoles (fluconazole or itraconazole) and/or amphotericin B desoxycholate; lipid formulations are too costly to be accessible[20].
THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).
The digestion step was repeated twice, and the 3 cell isolates were pooled in mouse osteoblast growth medium (OBGM), consisting of Dulbecco's modified Eagle's medium containing 25 mM HEPES, 10% fetal bovine serum (Sigma), 2 g of sodium bicarbonate per liter, 75 μg of glycine/ml, 100 μg of ascorbic acid/ml, 40 ng of vitamin B 12 /ml, 2 μg of p -aminobenzoic acid/ml, 200 ng of biotin/ml, and penicillin (100 U/ml)-streptomycin (100 μg/ml)-amphotericin B (25 μg/ml) (pH 7.4) [ 60 ] . Cells were seeded in six-well cluster plates and incubated at 37°C in a 5% CO 2 atmosphere until they reached confluence (6 to 7 days).
Briefly, the cells were mechanically dissociated, suspended in Opti-MEM with 5% heat-inactivated fetal bovine serum, 0.2% N2 supplement (GIBCO) and 1% antibiotic solution (penicillin G 10 4units/ml, streptomycin 10 mg/ml and amphotericin B 25 μg/ml) and plated in flat bottom 96 well plates.
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