Words similar to amplifying
Example sentences for: amplifying
How can you use “amplifying” in a sentence? Here are some example sentences to help you improve your vocabulary:
We verified the location of CARD15 on our map by amplifying a 444 bp fragment of exon 4 from clone 327F22 in our map (see additional data file 1, blau_map.pdf).
Two hybridization probes spanning the entire sarcospan coding sequence were generated by PCR amplification of sarcospan cDNA amplifying 366 bp (5'-ATGGGCAAGAACAAGCAGCCACG-3' and 5'-TTTCATAGAAAATTGAATACATGTCC-3') and 466 bp (5'-GGGCTGGGATCATTGTCTGCT-3' and 5'-GGAATTCTTAGATCTTTTGCTGGGG-3').
On each plate a standard curve was generated for both the GAPDH and target PCR reactions by amplifying 5 different known amounts of cDNA derived from total RNA.
Many of the discordant ratios in the R-PCR dataset likely result from a combination of the non-random fragmentation in the Alu I / Rsa I experiment and the ineffectiveness of R-PCR in amplifying low molecular weight species.
RT-PCR reactions using primers Hprt1a (5'-CCTGCTGGATTACATATAAGCACTG-3') and Hprt1b (5'-GTCAAGGGCATATCCAACAACAAAC-3') served as positive controls amplifying a 354 bp product from the ubiquitously expressed hprt transcript [ 15 ] .