Words similar to amplifying
Example sentences for: amplifying
How can you use “amplifying” in a sentence? Here are some example sentences to help you improve your vocabulary:
Many of the discordant ratios in the R-PCR dataset likely result from a combination of the non-random fragmentation in the Alu I / Rsa I experiment and the ineffectiveness of R-PCR in amplifying low molecular weight species.
Due to difficulties in amplifying the rabbit MYOC coding region from cDNA, we pieced together the entire contiguous open-reading frame using rabbit genomic DNA (Clontech; New Zealand White) as a template with a multi-step PCR/ligation process.
N-terminal deletions, in addition to the series obtained through two-hybrid screening, were constructed by amplifying the two-hybrid cDNA clones using primers containing Sma I and EcoR I at the N- and C-terminus, respectively, and inserting them into both pPC86 and pGEX-2TK vectors.
Baugh and colleagues were able to improve yields when amplifying mRNA by decreasing the concentration of T7 primer [ 16 ] . When we limited the mass of T7 primer adapter to approximately five times that of the starting material with a lower limit of 50 ng (by decreasing the second strand synthesis reaction volume), this low molecular weight species disappeared, without decreasing overall yields (data not shown).
Template was created by amplifying a band off an Expressed Sequence Tag (EST G 5 B 3 T 7 ) from the Arabidopsis expressed sequence tag database [ 78 ] , fractionating the product on a low melting point agarose gel, and excising the band.
Loading...