Example sentences for: amplifying

How can you use “amplifying” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Many of the discordant ratios in the R-PCR dataset likely result from a combination of the non-random fragmentation in the Alu I / Rsa I experiment and the ineffectiveness of R-PCR in amplifying low molecular weight species.

  • Due to difficulties in amplifying the rabbit MYOC coding region from cDNA, we pieced together the entire contiguous open-reading frame using rabbit genomic DNA (Clontech; New Zealand White) as a template with a multi-step PCR/ligation process.

  • N-terminal deletions, in addition to the series obtained through two-hybrid screening, were constructed by amplifying the two-hybrid cDNA clones using primers containing Sma I and EcoR I at the N- and C-terminus, respectively, and inserting them into both pPC86 and pGEX-2TK vectors.

  • Baugh and colleagues were able to improve yields when amplifying mRNA by decreasing the concentration of T7 primer [ 16 ] . When we limited the mass of T7 primer adapter to approximately five times that of the starting material with a lower limit of 50 ng (by decreasing the second strand synthesis reaction volume), this low molecular weight species disappeared, without decreasing overall yields (data not shown).

  • Template was created by amplifying a band off an Expressed Sequence Tag (EST G 5 B 3 T 7 ) from the Arabidopsis expressed sequence tag database [ 78 ] , fractionating the product on a low melting point agarose gel, and excising the band.


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