Words similar to mutagenized

Example sentences for: mutagenized

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Traditionally, structure-function analyses of RNA genomes, including those of RNA viruses, have relied on spontaneous mutants found in natural isolates or chemically mutagenized stocks [ 7 ] . Mutations in either case are essentially unpredictable and must be mapped by elaborate techniques such as classical complementation analyses or direct sequencing of the genome.
Mutagenesis of SWY2089 and SWY2090 with ethylmethane sulfonate (EMS; Sigma, St. Louis, MO) was performed as described with the following modifications [ 67 ] . First, cells were mutagenized for 60, 75, and 90 minutes, followed by plating a fraction of the culture to determine cell viability at 23°.
Wild type GFP-nic96 nup170-GFP haploid strains were mutagenized to generate a bank of ts mutant strains (Figure 1A; see Materials and Methods).
E. coli clones carrying the mutagenized plasmids were identified by colony lifts and each mutagenized plasmid was sequenced to confirm the mutations.
The bmr mutations were introduced via chemical mutagenesis with diethyl sulfate-ethylsulfonate (DES) [ 21 ] . Briefly, an M 3 population, obtained from selfing M 1 and M 2 plants (M 1 refers to plants grown from the mutagenized seeds) was screened for bmr mutants.