Words similar to mutagenized

Example sentences for: mutagenized

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Wild type GFP-nic96 nup170-GFP haploid strains were mutagenized to generate a bank of ts mutant strains (Figure 1A; see Materials and Methods).
Mutagenesis of SWY2089 and SWY2090 with ethylmethane sulfonate (EMS; Sigma, St. Louis, MO) was performed as described with the following modifications [ 67 ] . First, cells were mutagenized for 60, 75, and 90 minutes, followed by plating a fraction of the culture to determine cell viability at 23°.
The bmr mutations were introduced via chemical mutagenesis with diethyl sulfate-ethylsulfonate (DES) [ 21 ] . Briefly, an M 3 population, obtained from selfing M 1 and M 2 plants (M 1 refers to plants grown from the mutagenized seeds) was screened for bmr mutants.
The mutagenized residues probably are indeed important for the function of this protein, but not because they are part of a GCN5-like acetyltransferase domain, which this protein does not contain.
E. coli clones carrying the mutagenized plasmids were identified by colony lifts and each mutagenized plasmid was sequenced to confirm the mutations.