Words similar to mutagenized
Example sentences for: mutagenized
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The remaining mutagenized cell cultures were then plated to obtain ~100,000 colonies from each strain.
The bmr mutations were introduced via chemical mutagenesis with diethyl sulfate-ethylsulfonate (DES) [ 21 ] . Briefly, an M 3 population, obtained from selfing M 1 and M 2 plants (M 1 refers to plants grown from the mutagenized seeds) was screened for bmr mutants.
Mutagenesis of SWY2089 and SWY2090 with ethylmethane sulfonate (EMS; Sigma, St. Louis, MO) was performed as described with the following modifications [ 67 ] . First, cells were mutagenized for 60, 75, and 90 minutes, followed by plating a fraction of the culture to determine cell viability at 23°.
Traditionally, structure-function analyses of RNA genomes, including those of RNA viruses, have relied on spontaneous mutants found in natural isolates or chemically mutagenized stocks [ 7 ] . Mutations in either case are essentially unpredictable and must be mapped by elaborate techniques such as classical complementation analyses or direct sequencing of the genome.
The mutagenized residues probably are indeed important for the function of this protein, but not because they are part of a GCN5-like acetyltransferase domain, which this protein does not contain.